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Biological photosensitizer

A photosensitizer and biological technology, applied in the direction of drug combination, medical preparations of non-active ingredients, powder delivery, etc., can solve the problem of increased side effects of photodynamic therapy, poor photodynamic therapy effect, and poor absorption rate of non-targeted nanoparticles. advanced questions

Inactive Publication Date: 2016-03-02
CHINA UNIV OF PETROLEUM (EAST CHINA)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Non-targeted biological photosensitizers, because they have no specific recognition function for cancer cells, will also attack normal tissue cells, which will greatly increase the side effects of photodynamic therapy
At the same time, only when the biophotosensitizer enters the interior of the cell can it be more lethal to cancer cells, and the absorption rate of cancer cells to untargeted nanoparticles is not high
resulting in ineffective photodynamic therapy

Method used

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  • Biological photosensitizer

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Experimental program
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Effect test

Embodiment 1

[0019] Embodiment 1: the preparation of biological photosensitizer

[0020] 1) Amino groups on the surface of mesoporous silica-coated upconversion nanoparticles (NaYF4:Yb / Er):

[0021] Weigh 15 mg of mesoporous silica-coated upconverting nanoparticles (NaYF4:Yb / Er), add it to a vial containing 3 ml of toluene solution, seal it and rotate at 300 rpm for 6 hours at room temperature (the rotation speed should not be too fast, not exceeding 600 rpm) . Then add 50 ul of APTMS (volume ratio toluene:APTMS=60:1), and the solution is sealed and rotated at room temperature for 24 hours. After the rotation, the nanoparticles were collected by centrifugation at a relative centrifugal force of 3500 g for 6 minutes. Take 3ml of toluene solution, pipette the solution until the particles are evenly mixed, and the solution becomes a suspension, then centrifuge to collect the nanoparticles, the relative centrifugal force is 3500g, the time is 6min, repeat the above steps, wash with 3ml of to...

Embodiment 2

[0028] Embodiment 2: the preparation of biological photosensitizer

[0029] 1) Amino groups on the surface of mesoporous silica-coated upconversion nanoparticles (NaYF4:Yb / Er):

[0030] Weigh 30 mg of mesoporous silica-coated upconverting nanoparticles (NaYF4:Yb / Er), add it to a vial containing 3 ml of toluene solution, seal it and rotate it at room temperature at 300 rpm for 6 h (rotation speed should not be too fast, not exceeding 600 rpm) . Then add 50 ul of APTMS (volume ratio toluene:APTMS=60:1), and the solution is sealed and rotated at room temperature for 24 hours. After the rotation, the nanoparticles were collected by centrifugation at a relative centrifugal force of 3500 g for 6 minutes. Take 3ml of toluene solution, pipette the solution until the particles are evenly mixed, and the solution becomes a suspension, then centrifuge to collect the nanoparticles, the relative centrifugal force is 3500g, the time is 6min, repeat the above steps, wash with 3ml of toluene...

Embodiment 3

[0038] The biological photosensitizer of the invention can be used for efficient and strong targeted photodynamic therapy of deep tissue. The larger the wavelength of the light, the stronger the tissue penetration ability, and the near-infrared light can reach the deep layer of the tissue. β-NaYF 4 : Yb / Er up-conversion nanoparticles of this crystal phase can absorb near-infrared 980nm laser light and emit visible light with wavelengths of 520nm, 540nm and 653nm. Photodynamic therapy of deep tissue can be realized by loading photosensitizer on it.

[0039] The present invention loads phthalocyanine titanium dichloride and hyaluronic acid onto mesoporous silica-coated upconversion nanoparticles (NaYF 4 :Yb / Er), hyaluronic acid can specifically recognize cancer cells with overexpression of CD44, such as: colon cancer cells and breast cancer cells, etc., endowing the biophotosensitizer with high targeting. Titanium phthalocyanine dichloride, a photosensitizer, has a high energ...

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Abstract

The invention aims to provide a biological photosensitizer, namely a novel biological photosensitizer high in penetratability and targeting. Nanoparticles are modified with titanium phthalocyanine dichloride and hyaluronic acid in a suitable loading sequence and in an efficient combination manner, the modified up-converted nanoparticles are high in singlet oxygen yield and have a high targeting function of specific recognition for cells of over-expressed CD44 protein.

Description

technical field [0001] The invention belongs to the technical field of photosensitizer preparation, and in particular relates to a biological photosensitizer. Background technique [0002] The current problems in the field of biophotosensitizer preparation mainly include the low energy conversion efficiency between the loaded photosensitizer and nanoparticles, and thus the low yield of singlet oxygen of the biophotosensitizer. The important reason is related to the characteristics of the photosensitizer loaded on nanoparticles. After the general photosensitizer absorbs photons, the energy exchange rate with oxygen molecules is low, and the amount of singlet oxygen produced is small. General biophotosensitizers have no specific target on the target. Non-targeted biophotosensitizers, because they have no specific recognition function for cancer cells, will also attack normal tissue cells, which will greatly increase the side effects of photodynamic therapy. At the same time,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K9/14A61K47/04A61K47/36A61P35/00
CPCA61K41/0071A61K9/143A61K47/36
Inventor 姜磊燕照霞刘涵云杨丽敏马洪超
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
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