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Preparation method for whitmania pigra Whitman polypeptide zinc chelate possessing antibacterial activity

A technology of antibacterial activity and chelate, which is applied in the field of preparation of leech polypeptide zinc chelate, can solve the problems of environmental pollution, protein waste, etc., and achieve the effect of improving chelation rate and high yield

Inactive Publication Date: 2016-02-24
CHANGSHU INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some pharmaceutical companies, after extracting the medicinal ingredients in leech, discard the remaining leech tissue as medicinal residues, which not only pollutes the environment but also wastes a lot of protein

Method used

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  • Preparation method for whitmania pigra Whitman polypeptide zinc chelate possessing antibacterial activity
  • Preparation method for whitmania pigra Whitman polypeptide zinc chelate possessing antibacterial activity
  • Preparation method for whitmania pigra Whitman polypeptide zinc chelate possessing antibacterial activity

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Experimental program
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Effect test

Embodiment 1

[0041] Embodiment 1 The preparation method of the leech polypeptide zinc chelate with antibacterial activity of the present invention

[0042] (1) Degreasing of leeches wide-body tissue: Weigh 142g of leeches wide-bodied and cleaned, freeze to death at -80°C, homogenate and grind, and then add 99.4g isotropic acid containing 0.5% citric acid to the homogenate. Propanol (0.7 times the weight of the degreaser as the sample). The homogenate was put into a conical flask and defatted on a shaker for 12 hours. After degreasing, centrifuge, discard the supernatant and keep the precipitate, and freeze-dry the precipitate to obtain the degreasing product for future use.

[0043] (2) Enzymatic hydrolysis of the source protein of leech chinensis: mix 2g of the degreasing product of leech chinensis with 40ml of deionized water (the mass ratio of the degreasing product of leech chinensis and deionized water is 1:20), Then adjust the temperature to 40°C and the pH to 6.0, add 300,000U of ...

Embodiment 2

[0048] Embodiment 2 The preparation method of the leech polypeptide zinc chelate with antibacterial activity of the present invention

[0049] (1) Degreasing of leeches wide body tissue: Weigh 158g of cleaned leeches wide body, freeze to death at -80°C, homogenize and grind, then add 158g ethyl acetate containing 0.5% citric acid to the homogenate Esters (the weight of the degreasing agent is 1 times of the sample). The homogenate was put into Erlenmeyer flask and defatted on the shaker for 18h. After degreasing, centrifuge, discard the supernatant and keep the precipitate, and freeze-dry the precipitate to obtain the degreasing product for future use.

[0050] (2) Enzymatic hydrolysis of leech-derived protein: mix 2g of leech degreasing product with 20ml of deionized water (mass ratio of leech degreasing product to deionized water is 1:10), Then adjust the temperature to 46°C and pH to 8.0, add 260,000U enzyme-active flavor protease (add 130U enzyme-activated enzyme to ever...

Embodiment 3

[0055] Embodiment 3 The preparation method of the leech polypeptide zinc chelate with antibacterial activity of the present invention

[0056] (1) Degreasing of the wide-bodied leech tissue: Weigh 132g of the cleaned wide-bodied leech, freeze to death at -80°C, homogenize and grind, and then add 66g of isopropanol containing 0.5% citric acid to the homogenate Alcohol (the weight of the degreasing agent is 0.5 times that of the sample). The homogenate was put into a conical flask and defatted on a shaker for 24 hours. After degreasing, centrifuge, discard the supernatant and keep the precipitate, and freeze-dry the precipitate to obtain the degreasing product for future use.

[0057] (2) Enzymatic hydrolysis of the source protein of leech chinensis: mix 2g of the degreasing product of leech chinensis with 30ml of deionized water (the mass ratio of the degreasing product of leech chinensis and deionized water is 1:15), Then adjust the temperature to 50°C and the pH to 7.0, add...

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Abstract

The invention discloses a preparation method for a whitmania pigra Whitman polypeptide zinc chelate possessing antibacterial activity. The method comprises the following main steps: (1) degreasing whitmania pigra Whitman tissue; (2) performing enzymolysis on whitmania pigra Whitman protein; (3) separating whitmania pigra Whitman polypeptide; (4) performing chelation reaction on whitmania pigra Whitman polypeptide subjected to enzymolysis and zinc sulfate heptahydrate; and (5) performing alcohol precipitation separating and washing on the chelation reaction product. The prepared whitmania pigra Whitman polypeptide zinc chelate possesses relatively high yield and chelation rate, and oxford-cup-method bacteriostasis detection test proves that the whitmania pigra Whitman polypeptide zinc chelate possesses obvious antibiosis biological activity on escherichia coli and bacillus subtilis, and possesses great application prospect in the field of food and medicines.

Description

technical field [0001] The invention relates to the technical field of production of aquatic animal biological products, in particular to a preparation method of leech polypeptide zinc chelate with antibacterial activity. Background technique [0002] With the development of medical technology in recent years, antibiotics have become one of the medicines that people often use. With the more and more widespread application of antibiotics, a series of problems such as the abuse of antibiotics have emerged, which seriously threaten human life and health. At present, in the medical field all over the world, the irrational use and abuse of antibiotics is very serious, which directly leads to the strong drug resistance of some bacteria and reduces the efficiency of antibiotics. [0003] In the face of the increasing drug resistance of some bacteria, the most effective and direct way is to find a new type of antibiotic with strong antibacterial ability. Studies have shown that so...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/36C07K1/30C07K1/20
Inventor 韩曜平曾庆瑞朱敏佳王梦璠徐盛金狄鹏程周宇钱文举陈媛媛丁磊胡沁莹李嘉徐奕
Owner CHANGSHU INSTITUTE OF TECHNOLOGY
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