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Method of Tissue Culture and Rapid Propagation of Lvzhou No.1 Once Seedlings

A tissue culture rapid multiplication, No. 1 technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low seedling utilization rate, seedling damage, and high transportation costs

Active Publication Date: 2018-01-12
运城市绿碧源农林开发有限公司
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  • Abstract
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  • Claims
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AI Technical Summary

Problems solved by technology

The obvious disadvantage of this method is that the transportation cost is high, and the seedlings may be irreversibly damaged during transportation, resulting in low seedling utilization.

Method used

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Examples

Experimental program
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Embodiment Construction

[0012] 1. Obtaining explants: On a sunny day, select Lvzhou No. 1 plants with robust growth and no pests and diseases, cut the stem segments as explants, cut off the leaves, peel off the leaf sheaths, and cut them into about 2cm with alcohol-sterilized pruning shears Each segment has a node (axillary bud), 0.5cm below the node and 1.5cm above the node. The stem segment with nodes and axillary buds, without peeling off the axillary buds, the nutrition in the stem segment can promote the rapid growth of the axillary buds.

[0013] 2. Disinfection of explants: Treat with 75% alcohol for 30 seconds, then rinse with 80,000 units of sterile gentamicin solution for 3 times on an ultra-clean workbench, and disinfect with 1.5-2‰ of mercuric chloride5 - 10 minutes and shaking several times, and finally shake and wash 3 times with 80,000 units of gentamicin sterile solution;

[0014] 3. Preparation of culture medium: After all the medicines in the culture medium are dissolved and adjust...

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Abstract

The invention relates to a tissue culture and rapid propagation culture medium of Lvzhou No.1 grass. A culture medium formula is formed by combining a culture solution with a low salt concentration with a plant growth conditioning agent; by using the tissue culture and rapid propagation culture medium provided by the invention, an optimal plant axillary bud stem section is used as an explant; in a whole sterile culture process, only one culture medium is needed; the culture process is simple and feasible. In the culture medium, subculture and rooting induction are carried out synchronously and one-step seedling culture is realized. A seedling culturing period of Lvzhou No.1 grass tissue culture seedlings is shortened and industrial production is facilitated. The invention further relates to a one-step seedling culturing tissue culture and rapid propagation method of the Lvzhou No.1 grass; a multiplication culture process is a manner of multiplying the seedlings after the seedlings obtained by culturing stem section buds (with bud sections) in one step are chopped, and the multiplication coefficient is 3 to 5; no variation phenomenon is caused and no callus is formed in a rooting process; base parts of axillary buds are used for rooting and the transplanting survival rate is high.

Description

technical field [0001] The invention relates to a tissue culture fast propagation medium and a tissue culture fast propagation method for plants, in particular to a tissue culture fast propagation medium of Lvzhou No. Background technique [0002] The new Juncao technology was invented by Lin Zhanxi, a researcher at Fujian Agriculture and Forestry University and known as the "Father of Juncao in the World". This technology has successfully solved the worldwide problem of "contradiction between bacteria and forests", replacing wood with grass, turning waste into treasure, and making a huge contribution to solving human poverty and protecting the "lung" of human beings - forest. After nearly 30 years of innovative research and development, this technology has become increasingly mature. Nearly 60 species of weeds that can replace wood for cultivating fungi have been selected from thousands of species of weeds, and the research and application of Juncao has been extended to cul...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 王晓翔吴慎杰李静张换样焦改丽
Owner 运城市绿碧源农林开发有限公司
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