Medical applications of 20(S)-protopanoxadiol
A technology of protopanaxadiol and medicine, which is applied in the field of medical application of 20-protopanaxadiol, which can solve problems such as poor blood sugar control, cell reduction, and poor blood circulation
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[0033] 1. Experimental materials
[0034] 1.1 Medicinal materials
[0035] 20(S)-Protopanaxadiol (molecular weight: 460, HPLC purity ≥ 99%, Institute of Traditional Chinese Medicine)
[0036] 1.2 Experimental animals
[0037] db / db female mice (12 weeks) weighing 50±5 g were provided by the Experimental Animal Center of Shanghai University of Traditional Chinese Medicine, animal certificate number: SCXK (Shanghai) 2012-0002. Placed in a conventional feeding environment, housed in separate cages, free to eat and drink.
[0038] 2. Experimental method
[0039] 2.1 Establishment of diabetes injury model
[0040] Using the modeling method in the literature (Nature Protocols, 2013, 8:302-309), after isoflurane anesthesia, the back skin was shaved, depilatory cream was removed, and routine skin disinfection was used. A circular incision is made laterally, reaching deep to the fascia. To prevent automatic contraction of the wound, silicone gaskets (0.5 mm thick, inner diameter ...
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