Reproduction method for prunus wilsonii
The technology of a purple leaf thick plum and the propagation medium is applied in the field of purple leaf thick plum propagation, which can solve the problems that the purple leaf thick plum cannot be propagated on a large scale and the effect of the purple leaf thick plum is poor, so as to increase the application value and improve the greening effect , the effect of increasing productivity
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specific Embodiment approach 1
[0021] Specific embodiment one: a kind of purple leaf thick plum propagation method of the present embodiment is carried out according to the following steps:
[0022] 1. Explant selection:
[0023] Select the axillary buds at the end of April as explants; first wash them with clean water, soak them in 75% ethanol by volume for 30 seconds, take out the clean water and rinse them 3 to 5 times, and then soak them in 0.5% sodium hypochlorite by volume for 10 minutes , and then rinsed with sterile water 3 to 5 times;
[0024] 2. Induction of explant buds:
[0025] Cut the explants treated in step 1 into 1-2cm stem segments and place them in the explant bud induction medium at a temperature of 20-22°C, light intensity of 2300lx, light of 12-15h / d, and culture for 30d ; Wherein, the explant bud induction medium is MS+6-BA1.5mg / L+NAA0.5mg / L+KT0.5mg / L+IBA0.3mg / L+Vc400~500mg / L+Sm (NO 3 ) 3 20~25mg / L;
[0026] 3. Proliferation culture:
[0027] Place the induced tissue in step 2 i...
specific Embodiment approach 2
[0031] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the explant bud induction medium is MS+6-BA1.5mg / L+NAA0.5mg / L+KT0.5mg / L+ IBA0.3mg / L+Vc450mg / L+Sm(NO 3 ) 3 20~25mg / L. Others are the same as in the first embodiment.
specific Embodiment approach 3
[0032] Specific embodiment three: the difference between this embodiment and specific embodiment one is: the described proliferation medium is MS+Sm (NO 3 ) 3 40mg / L+NAA0.5mg / L+6-BA0.5mg / L+IBA0.5mg / L. Others are the same as in the first embodiment.
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