Reproduction method for prunus wilsonii

The technology of a purple leaf thick plum and the propagation medium is applied in the field of purple leaf thick plum propagation, which can solve the problems that the purple leaf thick plum cannot be propagated on a large scale and the effect of the purple leaf thick plum is poor, so as to increase the application value and improve the greening effect , the effect of increasing productivity

Inactive Publication Date: 2016-01-13
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] But at present, the effect of the method of breeding and cultivating Prunus japonica is poor, and it cannot effectively carry out large-scale propagation of Prunus japonica

Method used

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  • Reproduction method for prunus wilsonii
  • Reproduction method for prunus wilsonii
  • Reproduction method for prunus wilsonii

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0021] Specific embodiment one: a kind of purple leaf thick plum propagation method of the present embodiment is carried out according to the following steps:

[0022] 1. Explant selection:

[0023] Select the axillary buds at the end of April as explants; first wash them with clean water, soak them in 75% ethanol by volume for 30 seconds, take out the clean water and rinse them 3 to 5 times, and then soak them in 0.5% sodium hypochlorite by volume for 10 minutes , and then rinsed with sterile water 3 to 5 times;

[0024] 2. Induction of explant buds:

[0025] Cut the explants treated in step 1 into 1-2cm stem segments and place them in the explant bud induction medium at a temperature of 20-22°C, light intensity of 2300lx, light of 12-15h / d, and culture for 30d ; Wherein, the explant bud induction medium is MS+6-BA1.5mg / L+NAA0.5mg / L+KT0.5mg / L+IBA0.3mg / L+Vc400~500mg / L+Sm (NO 3 ) 3 20~25mg / L;

[0026] 3. Proliferation culture:

[0027] Place the induced tissue in step 2 i...

specific Embodiment approach 2

[0031] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the explant bud induction medium is MS+6-BA1.5mg / L+NAA0.5mg / L+KT0.5mg / L+ IBA0.3mg / L+Vc450mg / L+Sm(NO 3 ) 3 20~25mg / L. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0032] Specific embodiment three: the difference between this embodiment and specific embodiment one is: the described proliferation medium is MS+Sm (NO 3 ) 3 40mg / L+NAA0.5mg / L+6-BA0.5mg / L+IBA0.5mg / L. Others are the same as in the first embodiment.

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Abstract

The invention relates to a reproduction method for prunus wilsonii. The reproduction method includes the steps of explant selection, explant bud induction, multiplication culture, rooting culture and seedling hardening. As Sm element and Ce element are added into a tissue culture medium, the content of flavone, polyphenol and anthocyanin of prunus wilsonii is increased, the yield of prunus wilsonii extract is increased, and the application value of prunus wilsonii is increased.

Description

technical field [0001] The invention relates to a method for propagating purple leaf thick plum. Background technique [0002] Prunus virginiana, also known as Canadian red cherry, is a deciduous tree of Rosaceae (Rosaceae). It is native to North America and was bred from Prunus virginiana var.schubert in the 1950s. The skin is taupe, the branchlets are smooth and the tree shape is open naturally, the leaves are luxuriant, the leaves are oblong to obovate, 8-14cm long, 5-7cm wide, the margins are thickly toothed and short branches bloom, and the leaf axils produce racemes, the inflorescences are 4-6cm long , white flowers, purple-red fruit in flowering period from April to June, bitter taste, bright peel, well-developed root system in fruiting period from July to September, strong suitability, like sunshine and warm and humid climate environment, in fertile, loose and well-drained sandy soil It grows robustly on loam soil, has strong stress resistance, is resistant to cold ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 刘洪家张久明
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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