Microfluidic devices for single cell capture and three-dimensional rotation

Abstract

The invention discloses a microfluidic device used for single cell capture and three-dimensional rotation. The microfluidic device comprises a single cell capture device, a three-dimensional rotating device and a controller, wherein the single cell capture device is used for capturing single cells in cell suspension and comprises a first conductive substrate and a micro-channel; the three-dimensional rotating device is used for three-dimensionally rotating the captured single cells and comprises a second conductive substrate and a plurality of vertical electrodes, the vertical electrodes form an electrode cavity, and a three-dimensional rotation electric field is generated in the electrode cavity when power is on; the controller is used for controlling the flow speed and direction of the cell suspension in the micro-channel and applying electric signals to the first conductive substrate, the vertical electrodes and the second conductive substrate. The microfluidic device has the advantages that the single cells can be captured accurately, cell sample waste is avoided, and three-dimensional rotation of the cells can be achieved efficiently, fast and accurately.

Description

technical field [0001] The invention relates to the technical field of single cell rotation, in particular to a microfluidic device for single cell capture and three-dimensional rotation. Background technique [0002] At present, in the field of single-cell rotation, it is mainly to rotate a single cell in a two-dimensional plane, which is basically based on a planar electrode to operate a single cell. Two-dimensional rotation has certain limitations for cell research. First, it is impossible to control the precise Rotate the orientation of the cell, and secondly, it is impossible to fully understand the entire appearance of the cell. [0003] However, 3D rotation studies of cells are currently ineffective. For example, such as a new type of three-dimensional rotating structure of cells, the single-cell rotating structure designed by it can well realize the three-dimensional rotation of cells, and cells can rotate in a chamber surrounded by electrodes, but because the cell ...

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Problems solved by technology

However, the single-cell capture method in the related art has the following disadvantages when capturing: 1) the capture position of the cells cannot be accurately controlled; 2) the sample of cells in the required cell suspension should be large enough, resulting in a large cell sample size. Waste; 3) the geometric design of the flow channel is very complicated, which increases the preparation requirements and cost of the flow channel; 4) the design of the flow channel is very long, resulting in a large space occupied by each effective capture unit on the microfluidic chip, resulting in Great waste

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