Methods of sequencing the immune repertoire

Abstract

The invention provides a non-invasive technique for the detection and quantification of the immune repertoire, in a biological sample containing a plurality of distinct cell populations. Methods are conducted using sequencing technology to detect and enumerate immune repertoire within a heterogeneous biological sample.

Description

[0001] related application [0002] This application claims the benefit and priority of U.S. Provisional Application No. 61 / 806143, filed March 28, 2013, and U.S. Provisional Application No. 61 / 801785, filed March 15, 2013, the entire contents of which are incorporated by reference This article. technical field [0003] The present invention relates to the field of quantitative nucleic acid analysis. More specifically, the present invention provides methods for determining immune repertoires using high throughput sequencing. Background technique [0004] The hallmark of the adaptive immune response is the ability to generate a broad diversity of binding molecules, such as T cell antigen receptors and antibodies. Various molecular mechanisms can be used to generate initial diversity, including genetic recombination at multiple loci. Naive B cells and naive T cells are equipped with an initial repertoire of binding moieties that circulate at positions where they can come in...

AI Technical Summary

Problems solved by technology

[0007] Although reference is made to binding specificity, and indeed a large number of serological assays are based on the physical interaction between antigen and receptor, the underlying cause of the diversity lies in the gene sequences expressed by lymphocytes, which reflect the recombination that has been imposed on the cells Innumerable processes of variation and selection

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