Method for establishing male pure line of Oria tilapia

Abstract

The invention discloses a method for establishing an oreochromisco aureus male pure line. Purified male oreochromisco aureus ZZ and normally-used female oreochromisco aureus zw are mated, generated male oreochromisco aureus Zz and generated female oreochromisco aureus Zw through mating each account for 50%, and the oreochromisco aureus are bred to sexual maturity. The female oreochromisco aureus Zw with the Z chromosome from the purified male oreochromisco aureus are mated with gynogenesis male oreochromisco aureus ZZ again, generated fry are bred to sexual maturity, and thus a large number of pure line male oreochromisco aureus ZZ individuals are generated. By means of the method of parent selecting, ripening accelerating, gynogenesis, mating selecting and backcross, the good oreochromisco aureus male pure line can be established, and the merits of oreochromisco aureus as the male breeding parent can be effectively maintained.

Description

technical field [0001] The invention belongs to the germplasm improvement technology of oligo tilapia. In particular, the method can establish a pure male line of Oria tilapia by means of parental selection, ripening, gynogenesis, mating selection, and backcrossing. Background technique [0002] Oni hybrid tilapia (the offspring of the male parent of Oria tilapia mated with the female parent of Nile tilapia) is one of the main species of tilapia cultured in my country. After nearly 20 to 30 years of breeding, especially the parent The overuse of the over-utilization increases the genetic diversity of the parents. It is difficult to improve the genetic traits accurately through the traditional selection method of phenotypic traits, which leads to the existence of male rate, stress resistance and other traits in the produced Oni hybrid tilapia fry. There are different degrees of difference and differentiation, and there is a decline trend in the excellent traits of varieties. ...

AI Technical Summary

Problems solved by technology

[0002] Oni hybrid tilapia (the offspring of the male parent of Oria tilapia mated with the female parent of Nile tilapia) is one of the main species of tilapia cultured in my country. After nearly 20 to 30 years of breeding, especially the parent The overuse of the over-utilization increases the genetic diversity of the parents. It is difficult to improve the genetic traits accurately through the traditional selection method of phenotypic traits, which leads to the existence of male rate, stress resistance and other traits in the produced Oni hybrid tilapia fry. There are different degrees of difference and differentiation, and there is a decline trend in the excellent traits of varieties