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A kind of Italian stone pine seed viability assay method

A measurement method and a technology of viability, applied in the field of seedling breeding of excellent windproof and sand-fixing economic plants introduced from abroad, can solve problems such as long time-consuming, troublesome identification and judgment, and large differences in measurement results, so as to reduce inaccurate calculation and purchase cost input cost effect

Inactive Publication Date: 2017-08-18
GANSU DESERT CONTROL RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

We used to measure the viability of seeds through the germination rate of seeds, which took too long, about 1 month, and the measurement results varied greatly; we also used the potassium iodide determination method, which was time-consuming, soaking the seeds for 18 hours and placing them for 72 hours, and then staining, identification and judgment are more troublesome , and the measured result (46%) is lower than the actual emergence rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. seed introduction

[0031] In August 2012, 45kg of mature Italian stone pine seeds were imported from France through customs inspection. The thousand-grain weight of the seeds is 643.3g, and the water content is 3.6%.

[0032] 2 seed treatments

[0033] 2.1 Seed storage: Store Italian stone pine seeds in cotton bags, avoid smoke, rodent damage, insect damage, water humidity, etc. during storage; keep the room temperature at 13-30°C, and the relative humidity <30%.

[0034] 2.2 Impurity removal: Put the Italian stone pine seeds on the experimental bench, remove small stones, stones similar in size and color to the seeds and other debris, and remove the seeds that are obviously moldy and blackened. The Italian stone pine seeds are generally yellow.

[0035] 2.3 Water selection: Put the seeds to be tested in a large beaker and add distilled water to soak for 5 minutes, then use a glass rod to stir continuously for 3 minutes to stop, wait until the liquid surface is ...

Embodiment 2

[0051] 1. Seed introduction, using the seeds introduced in August 2012.

[0052] 2. Seed treatment

[0053] 2.1 Seed storage: with embodiment 1.

[0054] 2.2 Impurity removal: Same as Example 1.

[0055] 2.3 Water selection: Put the seeds to be tested in a large beaker and add distilled water to soak for 8 minutes, then use a glass rod to stir continuously for 4 minutes to stop, wait until the liquid surface is still for 12 minutes, and remove the seeds floating on the water surface. Then take out the seeds that sink to the bottom of the beaker and wait for the next step.

[0056] 2.4 Seed disinfection: completely immerse the water-selected Lycopodium pine seeds in HgCl with a mass percentage concentration of 0.1% 2 After 12 minutes in the reagent, take it out and rinse it 4 times with distilled water repeatedly.

[0057] 3. Seed coat abrogation

[0058] 3.1 Seed soaking

[0059] Randomly select 100 seeds from the sterilized seeds, soak them in distilled water for 6 hour...

Embodiment 3

[0071] 1. Seed introduction

[0072] In September 2013, 50kg of mature Italian stone pine seeds were imported from France through customs inspection. The thousand-grain weight of the seeds is 646.0g, and the water content is 3.9%.

[0073] 2. Seed treatment

[0074] 2.1 Seed storage: with embodiment 1.

[0075] 2.2 Impurity removal: Same as Example 1.

[0076] 2.3 Water selection: put the seeds to be tested in a large beaker and add distilled water to soak for 6 minutes, then use a glass rod to stir continuously for 5 minutes to stop, wait until the liquid surface is still for 15 minutes, and then proceed with the same as in Example 1.

[0077] 2.4 Seed disinfection: completely immerse the water-selected Lycopodium pine seeds in HgCl with a mass percentage concentration of 0.1% 2 After 15 minutes in the reagent, take it out and rinse it with distilled water for 5 times.

[0078] 3. Seed coat abrogation

[0079] 3.1 Seed soaking, same as Example 2.

[0080] 3.2 Broken sk...

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Abstract

The invention relates to the technical field of breeding seedlings of excellent windproof and sand-fixing economic plants imported from abroad, in particular to a method for measuring the viability of seeds of evergreen tree species Italian Lycopodium pine. The method includes seed introduction, seed treatment, seed coat breaking, seed soaking, TTC solution preparation, dyeing, identification and statistics. Specifically, the mature and plump Italian Lycopodium seeds imported from abroad were stored in cotton bags; the seeds were soaked in distilled water for 5-10 minutes in a beaker; 2 After soaking in the reagent for 10-15 minutes, soak in distilled water for 4-8 hours, dry the water with filter paper and break the skin; soak the seeds after breaking the skin, and then use 0.5% TTC solution dyeing agent to dye the seeds in an incubator; finally carry out identification and statistics . The method fills in the blank of the method for determining the viability of Italian stone pine seeds, and can accurately reflect the vitality of Italian stone pine seeds, thereby objectively and accurately calculating the seed sowing amount, thereby reducing the purchase cost and input cost of seeds.

Description

technical field [0001] The invention relates to the technical field of breeding seedlings of excellent windproof and sand-fixing economic plants imported from abroad, in particular to a method for measuring the viability of seeds of evergreen tree species Italian Lycopodium pine. Background technique [0002] Seed viability refers to the potential ability of the seed to germinate or the vitality of the embryo. Seed viability is not only an essential indicator in seed quality assessment, but also has important guiding significance for seedling cultivation and afforestation techniques. For example, people can accurately calculate the sowing amount by measuring the viability of seeds before sowing, thereby reducing the input cost of seeds (especially those imported or expensive seeds). In agricultural production, commonly used methods for measuring seed viability include seed germination method, TTC method, potassium iodide method, etc. These methods have many problems in act...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01C1/02A01C1/08
Inventor 刘有军刘世增满多清李得禄
Owner GANSU DESERT CONTROL RES INST
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