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Method for inducing vanda subconcolor leaves to obtain protocorm-like bodies

A technology similar to protocorm and Vanda orchid, applied in the field of plant reproduction, can solve the problems of scarcity of seeds and seedlings, inability to store nutrients, low natural reproduction rate, etc., and achieve the effect of realizing large-scale production, ensuring the quality of seedlings and ensuring the multiplication coefficient.

Inactive Publication Date: 2015-12-16
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The seeds of pure-color Vanda are small and cannot store nutrients. Under natural conditions, the seed germination stage completely relies on mycorrhizal fungi to provide nutrients. The natural reproduction rate is low, seedlings are scarce, and resource renewal is very difficult.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] An example of the method for protocorm-like induced protocorm-like leaves of pure color vanda of the present invention comprises the following steps:

[0014] (1) Acquisition of sterile leaves: Take pure-color Vanda blue sterile test tube seedlings, inoculate them on MS propagation medium, under the conditions of culture temperature 23-27°C, light intensity 1500lux, light time 6-8 hours / day Cultured for 60 days to obtain sterile clustered bud leaves, wherein 0.2mg / L of 6-benzyladenine 6-BA, 0.6mg / L of kinetin KT, 3.0mg / L of naphthaleneacetic acid NAA, 2.0mg The agar of / L polyvinylpyrrolidone PVP, 30g / L sucrose and 3.4g / L, the pH value of culture medium is 5.8;

[0015] (2) Leaves are induced to obtain protocorm-like: the aseptic clustered bud leaves obtained in step (1) are scratched with # on the back of the leaves with a scalpel and spread on the MS propagation medium at a culture temperature of 23-27 ℃, light intensity 1500lux, and light time 6-8 hours / day under th...

Embodiment 2

[0017] Another example of the method for protocorm-like induction of pure-color vanda leaves of the present invention comprises the following steps:

[0018] (1) Acquisition of sterile leaves: Take pure-color Vanda blue sterile test tube seedlings, inoculate them on MS propagation medium, under the conditions of culture temperature 23-27°C, light intensity 1500lux, light time 6-8 hours / day Cultured for 60 days to obtain sterile clustered bud leaves, wherein 0.2mg / L of 6-benzyladenine 6-BA, 0.6mg / L of kinetin KT, 3.0mg / L of naphthaleneacetic acid NAA, 2.0mg The agar of / L polyvinylpyrrolidone PVP, 30g / L sucrose and 3.4g / L, the pH value of culture medium is 5.8;

[0019] (2) Leaves are induced to obtain protocorm-like: the aseptic clustered bud leaves obtained in step (1) are scratched with # on the back of the leaves with a scalpel and spread on the MS propagation medium at a culture temperature of 23-27 ℃, light intensity 1500lux, and light time 6-8 hours / day under the condit...

Embodiment 3

[0021] Another example of the method for protocorm-like induced protocorm-like leaves of pure color vanda of the present invention comprises the following steps:

[0022] (1) Acquisition of sterile leaves: Take pure-color Vanda blue sterile test tube seedlings, inoculate them on MS propagation medium, under the conditions of culture temperature 23-27°C, light intensity 1500lux, light time 6-8 hours / day Cultured for 60 days to obtain sterile clustered bud leaves, wherein 0.2mg / L of 6-benzyladenine 6-BA, 0.6mg / L of kinetin KT, 3.0mg / L of naphthaleneacetic acid NAA, 2.0mg The agar of / L polyvinylpyrrolidone PVP, 30g / L sucrose and 3.4g / L, the pH value of culture medium is 5.8;

[0023] (2) Leaves are induced to obtain protocorm-like: the aseptic clustered bud leaves obtained in step (1) are scratched with # on the back of the leaves with a scalpel and spread on the MS propagation medium at a culture temperature of 23-27 ℃, light intensity 1500lux, and light time 6-8 hours / day und...

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Abstract

A method for inducing vanda subconcolor leaves to obtain protocorm-like bodies comprises the following steps: (1) inoculating an MS propagation culture medium with vanda subconcolor sterile test-tube seedlings for culturing, so as to obtain sterile cluster-bud leaves; (2) scratching the backs of the sterile cluster-bud leaves by using a scalpel, then, placing the scratched leaves in the MS propagation culture medium for culturing, and inducing the leaves to obtain the protocorm-like bodies. The vanda subconcolor protocorm-like bodies obtained by adopting the method are high in propagation coefficient and stout, robust plants are obtained after rejuvenation is carried out, and robust vanda subconcolor high-quality seedlings can be provided in a short period, so that the problem about large-scale seedling culture of the vanda subconcolor is effectively solved.

Description

technical field [0001] The invention relates to a plant propagation method, in particular to a method for inducing protocorm-like leaves from pure-color vanda orchid leaves. Background technique [0002] Pure color Vanda orchid (Vanda sub concolor T. Tanget F. T. Wang) is an epiphytic herbaceous plant belonging to the orchid family Vanda sub concolor. It belongs to tropical epiphytic orchids. It has strong drought resistance and is easy to cultivate. , growing on both sides of the upright stems, the inflorescences grow from the leaf axils, and can bloom in all seasons; if there is enough light, it can bloom 2-3 times a year; the flowering period is long, the flowers are colorful, and the flowers are unrestrained. It is a world-class flower Famous products, rich in high ornamental value. The seeds of pure Vanda orchids are small and cannot store nutrients by themselves. Under natural conditions, the seed germination stage completely relies on mycorrhizal fungi to provide nut...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 韦坤华王晓峰李林轩黄宝优肖冬缪剑华韦莹李翠王一诺韦范
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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