A phosphate-solubilizing and growth-promoting bacterium y40 of the genus Acinetobacter and its application

A bacterium and phosphorus-dissolving technology, which is applied in the field of agricultural microorganisms, can solve the problems of phosphorus-dissolving bacteria competition and difficulty in exerting activity, and achieve efficient phosphorus-dissolving and growth-promoting functions, good growth-promoting effects, and the effect of promoting crop growth

Active Publication Date: 2018-10-02
江苏绿博生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Phosphorus solubilizing bacteria in the rhizosphere of plants are not enough to compete with other microorganisms, and it is difficult to exert their activity

Method used

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  • A phosphate-solubilizing and growth-promoting bacterium y40 of the genus Acinetobacter and its application
  • A phosphate-solubilizing and growth-promoting bacterium y40 of the genus Acinetobacter and its application
  • A phosphate-solubilizing and growth-promoting bacterium y40 of the genus Acinetobacter and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, isolation and identification of functional strains

[0030] Cut 5g of potato roots into a triangular flask filled with 45mL of sterile water, vibrate at 170rpm for 30min to make a suspension, and then prepare a concentration of 10 -3 、10 -4 、10 -5 、10 -6 soil dilution. from 10 -4 、10 -5 、10 -6 Take 0.1mL of the diluted solution and spread it on the calcium phosphate salt inorganic phosphorus medium and the egg yolk medium respectively (each concentration is repeated 3 times on each medium). Separation and decomposition of organic phosphorus and inorganic phosphorus bacteria plates were cultured at 30°C for 2-3d and 5-7d, respectively, and were taken out for observation. The colony that produced a transparent circle on the solid medium plate was initially identified as phosphorus-solubilizing bacteria. After streaking and purification , stored in a refrigerator at 4°C for further study.

[0031] Wherein the preparation method of the calcium phosphat...

Embodiment 2

[0033] Embodiment 2, the production of functional bacteria filling bacteria suspension

[0034] Inoculate the strain Y40 (CGMCC NO.10241) into LB culture medium for liquid fermentation production. The conditions for fermentation production are: pH 6.0-7.0, fermentation temperature range 30-35°C, stirring speed 170-200 rpm, fermentation time 40h, so that the bacteria content in the fermentation broth is ≥1×10 10 cells / mL; the culture solution is the fermentation broth, centrifuged at 8000r / min for 6 minutes, and the bacteria are resuspended with an equal volume of 0.9% normal saline.

[0035]The preparation method of the LB medium used is as follows, taking the preparation of 1L medium as an example: peptone 10g, yeast powder 5g, NaCl 10g, agar 18-20g, constant volume to 1000mL, pH value natural, sterilized at 121°C for 20min.

Embodiment 3

[0036] Embodiment 3, greenhouse growth-promoting effect test

[0037] 3.1 Application effect of growth-promoting organic fertilizer on tomato (first season, second season)

[0038] The two-season tomato growth-promoting tests were carried out in the greenhouse of Jiangsu Zhongyi Biological Fertilizer Engineering Center. The test crop: the tomato variety is Hezuo 906. The tomato seeds were soaked in warm water at 60°C, stirred to room temperature, and the precipitated seeds were taken and sterilized with 5% sodium hypochlorite. After 3 minutes, wash with sterile water for 3-4 times, shading and accelerating germination in a 30°C incubator until it turns white, and then bury it in the substrate to cultivate timely, sufficient, strong and neat tomato seedlings. There are three treatments: 1) adding decomposed chicken manure, calcium phosphate and Y40 bacterial suspension (Y40); 2) adding decomposed chicken manure and calcium phosphate (CK1); 3) adding decomposed chicken manure an...

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Abstract

The invention provides an acinetobacter phosphate solubilizing growth-promotion bacterial strain Y40 and application thereof. The bacterial strain belongs to potato rhizosphere and is preserved in China General Microbiological Culture Collection Center on December 25, 2014 and the preservation number is CGMCC No.10241. Flask shaking test results show that the bacterial strain can effectively reduce the pH value of a solution and dissolve insoluble phosphorus in the solution; and bacterium filling pot experiment results show that the bacterial strain can effectively promote growth of tomato and eggplant plants, meanwhile, can colonize at rhizosphere of plants in large amount and lives in roots to play the effects of growth-promotion.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and provides a phosphorus-solubilizing and growth-promoting bacterium Y40 of the genus Acinetobacter and its application. Background technique [0002] Phosphorus is an essential nutrient element for plant growth. Phosphorus deficiency is common in cultivated land in my country. Ineffective phosphorus forms account for 95% of the soil, and plants can hardly absorb and utilize it directly. The insoluble phosphorus in the soil can be divided into organic phosphorus and inorganic phosphorus, and the insoluble organic phosphorus accounts for 20%-50% of the total phosphorus in the soil. The available phosphorus content of phosphate fertilizers such as superphosphate is low, and the seasonal utilization rate of phosphate fertilizers after application is 5%-25%, and most of the remaining phosphorus is mixed with Ca in the soil. 2+ , Fe 3+ and Al 3+ etc. combined to form insoluble phosphate,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05G3/00C12R1/01
Inventor 沈其荣李荣赵买琼张瑞福
Owner 江苏绿博生物科技有限公司
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