A phosphate-solubilizing and growth-promoting bacterium y40 of the genus Acinetobacter and its application
A bacterium and phosphorus-dissolving technology, which is applied in the field of agricultural microorganisms, can solve the problems of phosphorus-dissolving bacteria competition and difficulty in exerting activity, and achieve efficient phosphorus-dissolving and growth-promoting functions, good growth-promoting effects, and the effect of promoting crop growth
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Embodiment 1
[0029] Embodiment 1, isolation and identification of functional strains
[0030] Cut 5g of potato roots into a triangular flask filled with 45mL of sterile water, vibrate at 170rpm for 30min to make a suspension, and then prepare a concentration of 10 -3 、10 -4 、10 -5 、10 -6 soil dilution. from 10 -4 、10 -5 、10 -6 Take 0.1mL of the diluted solution and spread it on the calcium phosphate salt inorganic phosphorus medium and the egg yolk medium respectively (each concentration is repeated 3 times on each medium). Separation and decomposition of organic phosphorus and inorganic phosphorus bacteria plates were cultured at 30°C for 2-3d and 5-7d, respectively, and were taken out for observation. The colony that produced a transparent circle on the solid medium plate was initially identified as phosphorus-solubilizing bacteria. After streaking and purification , stored in a refrigerator at 4°C for further study.
[0031] Wherein the preparation method of the calcium phosphat...
Embodiment 2
[0033] Embodiment 2, the production of functional bacteria filling bacteria suspension
[0034] Inoculate the strain Y40 (CGMCC NO.10241) into LB culture medium for liquid fermentation production. The conditions for fermentation production are: pH 6.0-7.0, fermentation temperature range 30-35°C, stirring speed 170-200 rpm, fermentation time 40h, so that the bacteria content in the fermentation broth is ≥1×10 10 cells / mL; the culture solution is the fermentation broth, centrifuged at 8000r / min for 6 minutes, and the bacteria are resuspended with an equal volume of 0.9% normal saline.
[0035]The preparation method of the LB medium used is as follows, taking the preparation of 1L medium as an example: peptone 10g, yeast powder 5g, NaCl 10g, agar 18-20g, constant volume to 1000mL, pH value natural, sterilized at 121°C for 20min.
Embodiment 3
[0036] Embodiment 3, greenhouse growth-promoting effect test
[0037] 3.1 Application effect of growth-promoting organic fertilizer on tomato (first season, second season)
[0038] The two-season tomato growth-promoting tests were carried out in the greenhouse of Jiangsu Zhongyi Biological Fertilizer Engineering Center. The test crop: the tomato variety is Hezuo 906. The tomato seeds were soaked in warm water at 60°C, stirred to room temperature, and the precipitated seeds were taken and sterilized with 5% sodium hypochlorite. After 3 minutes, wash with sterile water for 3-4 times, shading and accelerating germination in a 30°C incubator until it turns white, and then bury it in the substrate to cultivate timely, sufficient, strong and neat tomato seedlings. There are three treatments: 1) adding decomposed chicken manure, calcium phosphate and Y40 bacterial suspension (Y40); 2) adding decomposed chicken manure and calcium phosphate (CK1); 3) adding decomposed chicken manure an...
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