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Seed production method for new lines of wide-shell-type ruditapes philippinarum

A technology of Philippine clams and new strains, which is applied in the field of seed production of Philippine clams, can solve the problems of artificial large-scale cultivation of Philippine clams with wide shells, low yield, instability, etc., and achieve no exogenous gene introduction , fast growth and large egg production

Active Publication Date: 2015-08-26
DALIAN OCEAN UNIV
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AI Technical Summary

Problems solved by technology

At present, although the wide-shell Philippine clam can be selected from natural populations, its output is low and unstable, and it is still impossible to carry out artificial large-scale cultivation of the wide-shell Philippine clam, which is far from meeting the market demand.

Method used

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  • Seed production method for new lines of wide-shell-type ruditapes philippinarum
  • Seed production method for new lines of wide-shell-type ruditapes philippinarum
  • Seed production method for new lines of wide-shell-type ruditapes philippinarum

Examples

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Effect test

Embodiment

[0022] First, 2,000 wild clams were collected from the Zhangzidao Ecological Reserve in Dalian. Measure the shell length and shell width of each Philippine clam, and use the EXCEL spreadsheet software to calculate the ratio of shell width to shell length, arrange all the ratios in descending order, and select the top 10% ratio The corresponding individual, as the first-generation broodstock of the broad-shell Philippine clam,

[0023] Using the nest design method, the first generation of broodstock was mated according to the ratio of 1 male clam to 3 female clams, and 11 paternal half-sib families and 33 female full-sib families were established. After 3 months of breeding Finally, the heritabilities of shell length and shell width of half-sibs of paternal half-sibs of clams with shell width were estimated by SPSS software to be 0.76 and 0.35, respectively (see Table 1). It was found that clams with wide shells gradually showed the shell shape characteristics of their parents...

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Abstract

The invention discloses a seed production method for new lines of wide-shell-type ruditapes philippinarum. The seed production method comprises following steps: collecting multiple ruditapes philippinarum out of natural population, measuring width and length of a shell of each one of ruditapes philippinarum, arraying ruditapes philippinarum in the manner from large to small according to ratios among shell widths and shell lengths, selecting individuals ranking the top 10% thereof as the first generation of spats, utilizing the first generation of spats to set up multiple wide-shell-type families, utilizing a multi-trait Blup breeding method to select families with breeding values ranking the top 20% of families, selecting individuals with ratios among shell widths and shell lengths ranking the top 10% as the second generation of spats in accordance with the above mode in these families and breeding the second generation of spats, hastening parturition and carrying out artificial insemination and the like in order to obtain the line of wide-shell-type ruditapes philippinarum.

Description

technical field [0001] The invention relates to a seed production method of Philippine clam, in particular to a seed production method of a new strain of Philippine clam with wide shell. Background technique [0002] Philippine clams (Ruditapes philippenarum) It is a wide-temperature and wide-saline species, mainly distributed in the coasts of the Pacific Ocean and the Indian Ocean. It is a global farmed shellfish, with an annual output of about 3.78 million tons in the world, accounting for about 23.5% of the production of bivalve aquatic animals (Ghiselli et al, 2012) , about 80% of which come from mainland China (Zhang Guofan & Yan Xiwu, 2010), and the market prospect is broad. However, at present, the output of Philippine clams in my country is unstable, the stress resistance is poor, and the economic traits are degraded year by year, which restricts the development of Philippine clam breeding industry. Breeding high-yielding, stress-resistant new varieties of Philippi...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCA01K61/54Y02A40/81
Inventor 霍忠明闫喜武张兴志刘辉杨凤张国范
Owner DALIAN OCEAN UNIV
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