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A method for early detection of wilt disease in bayberry

A technology for early detection and wilt disease, applied in the field of early detection of bayberry wilt disease, can solve problems such as ineffectiveness

Active Publication Date: 2017-06-30
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At this time, if the application of pesticides is used for prevention and treatment, it will not have a good effect

Method used

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  • A method for early detection of wilt disease in bayberry
  • A method for early detection of wilt disease in bayberry
  • A method for early detection of wilt disease in bayberry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0018] 1 Materials and methods

[0019] 1.1 Sample collection and pretreatment

[0020] 1.2 Reagents

[0021] The PDA medium used for the isolation and purification of fungi was purchased from Qingdao Rishui Biotechnology Co., Ltd. The Taq DNA polymerase, dNTPs (mixture of 4 types) and 10×PCR buffer used in PCR amplification were purchased from Beijing Dingguo Biotechnology Co., Ltd., and the specific primers for P. , SYBR fluorescent reagents, pMD18-T vectors and competent cells used in real-time fluorescent quantitative PCR were purchased from Bao Biological Engineering (Dalian) Co., Ltd. The reagents required for genome extraction by CTAB method were purchased from Shanghai Sangon Bioengineering Co., Ltd., and the specific reagents are shown in the table below.

[0022] Table 1 Names and manufacturers of experimental reagents

[0023]

[0024]

[0025] 1.3 Instruments

[0026] Low temperature centrifuge (Eppendorf5417R); normal temperature centrifuge (Eppendorf54...

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Abstract

The invention relates to a method for early detection of whether wilt disease occurs in red bayberry, which is characterized in that the method includes: quantitatively amplifying the DNA of red bayberry tissue, if the heterochromatic pseudo-disc can be detected in every 100 ng of plant tissue DNA If the copy number of Polychaetes or / and Microsporum is greater than 1X106, it means that the bayberry tree wilt will definitely occur in the future. / and the copy number of Trichosporum microsporum is 1X105 smaller, it means that wilt disease will not occur in the bayberry tree in the future. Using the method of the present invention, it is possible to get up early to identify whether the bayberry tree has the risk of wilting disease, and to predict the risk of wilting disease of bayberry tree through the number of DNA copies, to predict the occurrence of wilting disease in advance, and to take timely and effective measures Prevention.

Description

technical field [0001] The invention belongs to the field of plant disease diagnosis, in particular to a method for early detection of waxberry wilt. Background technique [0002] Waxberry (Myrica rubra Sieb.Et Zucc.) is a rare fruit unique to southern my country. The fruit is sweet and sour and has a unique flavor. It enjoys a high reputation at home and abroad. The output value has ranked first among all kinds of fruits. Myrica rubra wilt is a newly discovered disease in recent years. At the beginning of the disease, some young shoots of bayberry are dry. As the disease worsens, the number of dry shoots gradually increases and spreads to the whole tree. The whole plant dies 3-5 years after the onset. Myrica rubra wilt is spreading rapidly in the main producing areas of bayberry in Zhejiang Province, seriously affecting the sustainable development of bayberry industry (Qiu Yingying et al. 2011). The pathogenic bacteria of bayberry wilt were Pestalotiopsis versicolor and P....

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04C12R1/645
Inventor 任海英戚行江梁森苗郑锡良张振兰朱潇婷
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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