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A kind of microalgae composite living bait for raising shellfish seedlings and its production method

A production method and technology of microalgae, applied in the field of microalgae composite living bait, can solve the problems of unstable culture environment, easy to be polluted, low degree of industrialization, etc., to improve growth rate and quality, avoid nutrient loss, and reduce morbidity Effect

Active Publication Date: 2018-03-06
JIANGSU UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the unique growth characteristics of microalgae, the industrialization degree of its large-scale production is still very low. So far, only the design and application of the open culture system have been relatively successful, which has been enlarged to 180,000 m in Taiwan, China. 2 (production of chlorella) and 200,000 m2 of Mexico City for the production of spirulina 2 , up to 50 hectares for Western Australia Biotech
However, open systems generally have disadvantages such as difficult to control growth parameters, unstable culture environment, easy to be polluted, difficult to achieve aseptic culture, and low yield. The cultivation of microalgae that tolerates growth in extreme environments; the large-scale cultivation of economical algae such as Diagonal hairs and golden algae is difficult to apply, which greatly restricts the development and marketization of microalgae bait products

Method used

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  • A kind of microalgae composite living bait for raising shellfish seedlings and its production method
  • A kind of microalgae composite living bait for raising shellfish seedlings and its production method
  • A kind of microalgae composite living bait for raising shellfish seedlings and its production method

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Embodiment

[0023] (1) According to the nutritional requirements of Pacific oyster seedlings (eye-spot larvae stage), Chaetoceros moowii, Coccolithophora, Isoflagellates, Haematococcus pluvialis, Chlorella pyrenoidosa and subcardioid planus were selected. Algae are species of microalgae.

[0024] (2) Using a photobioreactor (ZL201220434882.9) to cultivate the microalgae selected in step (1) to obtain a microalgae liquid. Among them, the culture temperature of Chaetoceros mouchii, Coccolithophora, Isoflagellates, Haematococcus pluvialis and P. 4 cells / mL, and the culture media were f / 2, NMB3, f / 2, BBM and Comvitamin; 4 cells / mL, and the medium was BG11 medium. After being cultured in a photobioreactor, the density of 1.0×10 9 (Chaetoceros moowii), 2.7×10 10 (coccolithophores), 1.5×10 10 (Isochrysis globosa), 6.8×10 9 (Haematococcus Pluvialls), 5.0×10 10 (Chlorella pyrenoidosa), 9.2×10 9 (Subcordiform Algae) cells / mL of microalgae liquid.

[0025] (3) The microalgae liquid obtained...

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Abstract

The invention discloses a microalgae composite living bait for raising shellfish seedlings and a production method thereof. The microalgae composite living bait is composed of the following microalgae species cultured and mixed in proportion to the number of cells: Chaetoceros mourii 15%‑20%, Coccolithia 20%‑25%, Isochrysis globosa 5%‑10%, Haematococcus pluvialis 15%‑20%, Chlorella pyrenoidosa 25%‑ 30% and P. subcordiformis 10%‑15%, with a total density of 1×105‑5×105 cells / mL. The production method is as follows: (1) use a photobioreactor to culture the microalgae species separately to obtain a microalgae liquid with a culture density of 1×109-5×1010 cells / mL; (2) prepare the microalgae liquid The microalgae composite living bait is formulated according to the ratio of the number of cells, and the microalgae composite living bait with a total density of 1×105-5×105 cells / mL is obtained. In the present application, a variety of microalgae cells are made into compound living bait, which has balanced nutrition, can effectively improve the survival rate of shellfish seedlings, and reduce the incidence rate thereof.

Description

technical field [0001] The invention belongs to the field of microalgae biotechnology, and in particular relates to a microalgae composite live bait for raising shellfish seedlings and a production method thereof. Background technique [0002] The aquaculture of economic shellfish such as oysters, mussels, clams and scallops accounts for an increasing share of world fisheries production and has become one of the pillars of the marine living resource industry. As the best bait for shellfish seedlings, microalgae is the only suitable food source for shellfish seedlings. [0003] In recent years, with the rapid development of the industrialization process of the aquaculture industry in various countries, the production technology of microalgae bait has developed rapidly, and the application and development of microalgae in aquaculture feed have developed rapidly. The proportion of microalgae bait in aquaculture feed Also getting bigger and bigger. In the production process of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K50/80A23K10/30
Inventor 邓祥元高坤成婕胡小丽
Owner JIANGSU UNIV OF SCI & TECH
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