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A kind of composite microbial decomposing bacterial agent, preparation method and application

A technology of compound microorganisms and decomposed inoculants, applied in the field of microbial inoculants and crop straw treatment, can solve problems such as soil ecosystem deterioration, impact on agricultural production, fire hazards, etc., and achieve good degradation ability, good synergistic effect, eliminate growth inhibitory effect

Active Publication Date: 2018-05-18
杨凌绿都生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] my country is a large agricultural country with 1.5 billion mu of arable land in the world. With the use of chemical fertilizers and pesticides, it has made a huge contribution to solving the problem of food and clothing for the world's population. At the same time, my country produces more than 700 million tons of crop straw every year, and the currently processed The main method is incineration, which not only causes serious air pollution and numerous fire hazards, but also deteriorates the soil ecosystem and affects agricultural production.

Method used

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  • A kind of composite microbial decomposing bacterial agent, preparation method and application
  • A kind of composite microbial decomposing bacterial agent, preparation method and application
  • A kind of composite microbial decomposing bacterial agent, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Preparation of straw returning decomposing agent with straw degradation effect,

[0026] (1) Fermentation tank culture solution formula: beef juice 0.5%, peptone 0.5%, NaCl 0.5%, soybean oil 0.5%, glucose 0.5%, magnesium sulfate 0.08%, potassium dihydrogen phosphate 0.04%, calcium carbonate 0.5%, 800 μm of Cu , Zn, Mn, Fe, stirred, sterilized, the measured pH value is 7-8.

[0027] (2) Training process

[0028] Its characteristic is that each strain is cultured and fermented separately, and after fermentation and subsequent processing are completed in the fermenter, they are mixed in proportion.

[0029] Inoculate Bacillus amyloliquefaciens Ba168, Paenibacillus pierreus BC-39 strain, streptomycin griseus and Candida krusei by streaking on beef juice peptone medium, culture at 28-30°C for 48 hours, and then respectively Insert into a 500mL Erlenmeyer flask, and incubate for 18h at 30°C and 180r / min. Then insert into 15L seed tanks respectively according to 1% inoculum...

Embodiment 2

[0033] Microbial Degradation Test of In-Situ Ground Covering Microbial Degradation of Corn Stalks of Straw Degradation Microbial Decomposition Preparation of the Present Invention

[0034] 1. Test materials

[0035] 1. Experimental site: located in the experimental field of Dazhai Township, Yangling District, Shaanxi Province. The experimental field has been planted with corn and wheat year after year, and the soil conditions are consistent. The degradation of the straw currently produced is all natural degradation, and the degradation is slow.

[0036] 2. Test combination:

[0037]The first group of compound microbial bacterial agents of the present invention Example 1, and the second group are microbial single bacterial agents No. 1, No. 2, and No. 3, each of which only includes Bacillus amyloliquefaciens Ba168, Paenibacillus BC-39, Kazakhstan Trichoderma twig components, the third group is the control group, which is routinely degraded naturally.

[0038] 2. Basic situat...

Embodiment 3

[0048] 1. Test materials

[0049] 1. Experimental site: located in the experimental field of Dazhai Township, Yangling District, Shaanxi Province. The experimental field has been planted with corn and wheat year after year, and the soil conditions are consistent. The degradation of the straw produced at present is all natural degradation, and the degradation is slow.

[0050] 2. Test drug:

[0051] Drugs to be tested: the first group is the compound microbial agent of Example 1 of the present invention, the second group is the microbial single agent, which only includes the Bacillus amyloliquefaciens Ba168 component, and the third group is the control group, which is conventionally degraded naturally.

[0052] 2. Basic situation:

[0053] The experiment was divided into three treatment groups: the experiment was carried out under field conditions, and the area of ​​each plot was 20m 2 . When the corn is harvested, the corn stalks are crushed, (15kg / area, equivalent to 500k...

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Abstract

The invention relates to compound microbial decomposed inoculas, and a preparation method and an application thereof. Effective components of the involved compound microbial decomposing inocula comprise bacillus amyloliquefaciens Ba168, paenibacillus peoriae BC-39, trichoderma harzianum, candida krusei, aspergillus niger and streptomyces griseus. The preparation method comprises that each strain are fermented separately, and the strains are mixed for use according to the proportion after the completion of the fermentation. The involved application is an application of the compound microbial decomposing inocula in degradation of crop straw. The compound microbial decomposing inocula not only give full play to unique degradation function of the bacillus on the straw, are combined with microbial compound flora for efficient synergy in the straw degradation process, thereby efficiently and rapidly degrading the straw, and significantly enhancing soil organic matter and humus levels.

Description

technical field [0001] The invention belongs to the technical field of crop stalk treatment and the field of microbial bacterial agents, and in particular relates to the preparation of a decomposing agent for returning corn stalks to the field with the function of degrading corn stalks. Background technique [0002] my country is a large agricultural country with 1.5 billion mu of arable land in the world. With the use of chemical fertilizers and pesticides, it has made a huge contribution to solving the problem of food and clothing for the world's population. At the same time, my country produces more than 700 million tons of crop straw every year, and the currently processed The main method is incineration, which not only causes serious air pollution and numerous fire hazards, but also deteriorates the soil ecosystem and affects agricultural production. Contents of the invention [0003] Aiming at the defects or deficiencies of the prior art, the invention provides a comp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/16C12N1/14C05F17/00C12R1/07C12R1/885C12R1/72C12R1/685C12R1/545C12R1/01
CPCY02W30/40
Inventor 杨轩付强杨长锁安德荣
Owner 杨凌绿都生物科技有限公司
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