A kind of preparation method of photosynthetic bacteria fertilizer
A technology of photosynthetic bacterial fertilizer and purple sulfur photosynthetic bacteria, which is applied in the field of photosynthetic bacterial fertilizer preparation, can solve the problems of open technical scheme of the preparation method of Thiobacillus pinkum, unfavorable large-scale application, and insignificant direct degradation, etc., and achieves enhanced tolerance Oxygen capacity and growth capacity, fast degradation rate of hydrogen sulfide, shortening the effect of culture time
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Embodiment 1
[0032] A kind of preparation method of photosynthetic bacteria fertilizer, adopts the Sulfur japonica strain in the purple sulfur photosynthetic bacteria to cultivate according to the following steps:
[0033] (1) Activation culture of semi-solid seeds: puncture the Sulfur japonica strain in the semi-solid purple sulfur photosynthetic bacteria culture medium, and culture it under light at 30°C for 7 days. strains of bacteria;
[0034] (2) Seed culture: inoculate the activated strains into the seed liquid medium at a temperature of 30°C, light intensity of 2000 lux, and light anaerobic culture for 7 days. The OD650 of the detected seeds is 1.4, and the number of viable bacteria is 800 million cfu / ml is the seed culture solution;
[0035] (3) Fermentation culture: Inoculate the seed culture liquid and the fermentation medium at an inoculum ratio of 1:5, culture anaerobically in a light culture tank for 8 days, the culture temperature is 30°C, the light intensity is 3000 lux, a...
Embodiment 2
[0040] A kind of preparation method of photosynthetic bacteria fertilizer, adopts the Sulfur japonica strain in the purple sulfur photosynthetic bacteria to cultivate according to the following steps:
[0041] ⑴. Activation culture of semi-solid seeds: puncture the Sulfur japonica strain in the semi-solid purple sulfur photosynthetic bacteria culture medium, and cultivate it under light at 30°C for 7 days. Strains;
[0042] (2) Seed culture: inoculate the activated strains into the seed liquid culture medium, the temperature is 32°C, the light intensity is: 3000lux, light anaerobic culture for 7 days, the OD650 of the detected seeds is 1.3, and the number of viable bacteria is 650 million cfu / ml is the seed culture solution;
[0043] (3) Fermentation culture: Inoculate the seed culture liquid and the fermentation medium with an inoculum ratio of 1:4, culture anaerobically in a light culture tank for 7 days, the culture temperature is 30°C, the light intensity is 3000 lux, an...
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