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Detection of immunoglobulin light chain restrication by rna in situ hybridization

A technique of immunoglobulin and in situ hybridization, applied in the filed U.S. Provisional Application Serial No. 61

Inactive Publication Date: 2015-05-13
ADVANCED CELL DIAGNOSTICS INC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, autoradiographic signals take two weeks or more to develop (Segal et al., supra, 1994), and this delay is unacceptable for clinical control

Method used

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  • Detection of immunoglobulin light chain restrication by rna in situ hybridization
  • Detection of immunoglobulin light chain restrication by rna in situ hybridization
  • Detection of immunoglobulin light chain restrication by rna in situ hybridization

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Embodiment

[0120] Detection of Immunoglobulin Light Chain Restriction in B Cells Using In Situ Hybridization

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Abstract

The invention provides a method for detecting immunoglobulin light chain restriction and clonality in B cells by obtaining a sample of B cells from a subject; conducting a duplex in situ hybridization assay on the sample using (i) at least one probe set which is designed to specifically hybridize to immunoglobulin kappa chain constant region (IGKCR) RNA; and (ii) at least one probe set which is designed to specifically hybridize to immunoglobulin lambda chain constant region (IGLCR) RNA; detecting signal associated with hybridized IGKCR probe and signal associated with hybridized IGLCR probe in a population of B cells in the sample; and determining a pattern of signal associated with hybridized IGKCR probe and hybridized IGLCR probe within individual cells in the B cell population, wherein the pattern of signal within individual cells indicates the presence or absence of light chain restriction and clonality of the B cells.

Description

[0001] This application claims priority to U.S. Application Serial No. 13 / 843408, filed March 15, 2013, and U.S. Provisional Application Serial No. 61 / 620634, filed April 5, 2012, and 2012 Priority to U.S. Provisional Application Serial No. 61 / 717064, filed October 22, each of which is hereby incorporated by reference in its entirety. [0002] This invention was made with government support under Grant No. R43CA168019 awarded by the National Cancer Institute. The government has certain rights in this invention. Background technique [0003] The present invention relates generally to in situ hybridization assays, and more particularly to assays for diagnosing B cell neoplasms. [0004] Immunoglobulins are composed of two heavy chains and two light chains. During B-cell differentiation, DNA encoding immunoglobulin genes undergoes rearrangements to produce a unique combination of immunoglobulin domains with a greater diversity of possible immunoglobulins available to an individ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC07K16/00C12Q1/6881C12Q1/6886C12Q2600/158C07K2317/515C12Q2600/112
Inventor 马小骏罗宇龄雷蒙德·塔布斯
Owner ADVANCED CELL DIAGNOSTICS INC
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