A Toxicity Evaluation Method of Haze Pollutants

An evaluation method and pollutant technology, applied in the field of environmental toxicology, can solve the problems of difficult operation, low accuracy, and single evaluation content, and achieve the effects of great flexibility, convenient material collection, and exclusion of influence

Inactive Publication Date: 2017-09-26
HANGZHOU NORMAL UNIVERSITY
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the deficiencies of the existing atmospheric particulate matter cytotoxicity evaluation method, and propose a haze pollutant toxicity evaluation method, which can make up for the existing evaluation method. Single, low accuracy and other disadvantages

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A Toxicity Evaluation Method of Haze Pollutants
  • A Toxicity Evaluation Method of Haze Pollutants
  • A Toxicity Evaluation Method of Haze Pollutants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0027] The present invention is further analyzed below in conjunction with specific examples.

[0028] As shown in the figure, the inventive method comprises the following steps:

[0029] Step (1). Inoculate the RTE rat tracheal epithelial cell line into the F12 culture medium containing fetal bovine serum, and place it in a sterile cell incubator at 37°C for 3 to 4 days; inoculate the cultured cells into the culture medium dish, inoculate 5×10 per dish 6 ~8×10 6 rat tracheal epithelial cells;

[0030] The F12 culture solution containing fetal bovine serum contains fetal bovine serum with a volume content of 3-7%, preferably a volume content of 5%;

[0031] The petri dish has a diameter of 5 cm and a height of 1 cm, and needs to be sterilized before use;

[0032] Step (2). Then in step (1), the cell culture dish is placed in the atmospheric particle collection device placed in the cell culture medium in the haze area, and the cells are poisoned at the temperature required ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
heightaaaaaaaaaa
Login to view more

Abstract

The invention discloses a method for evaluating the toxicity of haze pollutants. The method is to inoculate and culture the RTE rat tracheal epithelial cell line, and inoculate it into a culture dish; place the cell culture dish in a cell culture medium atmospheric particle collection device to infect the cells; at the same time, collect the particles in the haze area, Detect the particle size, heavy metal concentration, and other organic and inorganic components, and detect the concentration of PM10 and PM2.5 in the air; put the poisoned cells into the cell impedance measuring instrument, and test their cell recovery ability, chemotaxis migration ability and Anti-invasion ability; comprehensive evaluation and comparison of the toxic effects of different types of haze particles on rat tracheal epithelial cells in different haze areas. Compared with the traditional toxicity evaluation method, the ECIS technology used in the present invention has greater flexibility, the obtained data changes in real time, and the meaning is richer; the material is convenient, and the influence of individual differences on the experiment can be eliminated, and no ethics are involved. question.

Description

technical field [0001] The invention belongs to the field of environmental toxicology and relates to a method for evaluating the toxicity of haze pollutants. Background technique [0002] In recent years, smog incidents have occurred frequently in China. Studies have shown that there is a positive correlation between smog and respiratory diseases, and every 10ug / m increase in PM2.5 in Shanghai 3 , the day and lag 1d (lag0 ~ 1) respiratory disease mortality increased by 0.95%. The main components of smog are sulfur dioxide, nitrogen oxides and inhalable particulate matter. Among them, inhalable particles are particles with a particle size between 2.5 and 10 microns. These particles can enter the upper respiratory tract, especially particles with a particle size less than or equal to 2.5 microns. Most of them can pass through the human bronchus, reach the lungs, and even enter the human blood circulation , leading to the occurrence of respiratory diseases. Therefore, under...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/02
Inventor 张杭君方文迪王彬浩曾少媛
Owner HANGZHOU NORMAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap