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Method for extracting abelmoschus esculentus polysaccharide peptide from abelmoschus esculentus

A technology of polysaccharide peptide and okra, applied in the field of preparation of okra polysaccharide peptide, to achieve the effect of less waste, low production cost and high purity

Inactive Publication Date: 2015-04-08
TIANJIN INT JOINT ACADEMY OF BIOTECH & MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies on extracting glycopeptides from okra

Method used

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  • Method for extracting abelmoschus esculentus polysaccharide peptide from abelmoschus esculentus
  • Method for extracting abelmoschus esculentus polysaccharide peptide from abelmoschus esculentus

Examples

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Effect test

Embodiment 1

[0019] A method for extracting okra polysaccharide peptide from okra, characterized in that it comprises the following steps:

[0020] (1) select fresh okra fruit, clean it with water, and crush it with a crusher;

[0021] (2) The okra fruit after crushing is heated and refluxed with 8 mass times of 90% ethanol aqueous solution for extraction for 3 hours; filtered, the filter residue is extracted with 8 mass times of purified water for 2 hours, extracted twice, filtered, and combined filtrate Concentrate under reduced pressure to a liquid 1 with a relative density of 1.10 at 25°C;

[0022] (3) Carry out alcohol precipitation with 95% ethanol aqueous solution with a volume concentration of 3 times of the volume of the liquid, filter, and wash the precipitate with 95% ethanol aqueous solution for 2 times; then wash 2 times with acetone; then wash 2 times with ether time; vacuum drying, dissolving the dry matter in distilled water to prepare a solution with a concentration of 15...

Embodiment 2

[0025] A method for extracting okra polysaccharide peptide from okra, characterized in that it comprises the following steps:

[0026] (1) select fresh okra fruit, clean it with water, and crush it with a crusher;

[0027] (2) The crushed okra fruit is heated and refluxed with 6 mass times of 95% ethanol aqueous solution for extraction for 2 hours; filtered, the filter residue is heated and refluxed with 6 mass times of pure water for 3 hours, extracted once, filtered, and the filtrates combined Concentrate under reduced pressure to a liquid 1 with a relative density of 1.06 at 25°C;

[0028] (3) Carry out alcohol precipitation with 95% ethanol aqueous solution whose volume concentration is 4 times of the volume of the liquid, filter, and wash the precipitate with 95% ethanol aqueous solution for 1 time; then wash 1 time with acetone; then wash 1 time with ether time; vacuum drying, dissolving the dry matter in distilled water to make a solution with a concentration of 20g / L,...

Embodiment 3

[0031] A method for extracting okra polysaccharide peptide from okra, characterized in that it comprises the following steps:

[0032] (1) select fresh okra fruit, clean it with water, and crush it with a crusher;

[0033] (2) The crushed okra fruit is heated and refluxed for 4 hours with 85% ethanol aqueous solution with a volume concentration of 10 times by mass; filtered, and the filter residue is extracted for 1 hour with 10 times by mass of pure water heated and refluxed, extracted 3 times, filtered, and the filtrate combined Concentrate under reduced pressure to a liquid 1 with a relative density of 1.12 at 25°C;

[0034](3) Carry out alcohol precipitation with 95% ethanol aqueous solution whose volume concentration is 2 times of the volume of the liquid, filter, and the precipitation is washed 3 times with 95% ethanol aqueous solution; then wash 3 times with acetone; then wash 3 times with ether time; vacuum drying, dissolving the dry matter in distilled water to make ...

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Abstract

The invention discloses a method for extracting abelmoschus esculentus polysaccharide peptide from abelmoschus esculentus. The method comprises the following steps: (1) selecting fresh abelmoschus esculentus fruits and cleanly washing; crushing the abelmoschus esculentus fruits by a crushing machine; (2) heating, reflowing and extracting the crushed abelmoschus esculentus fruits with an ethanol water solution; filtering, and heating, reflowing and extracting filter residues with pure water; filtering; combining filtrates, decompressing and concentrating to obtain liquid 1; (3) carrying out alcohol precipitation with the ethanol water solution; filtering and washing sediment; drying in vacuum and dissolving a dry matter into distilled water to prepare a solution; dialyzing and concentrating dialysate to obtain liquid 2; carrying out alcohol precipitation; filtering and washing sediment with ethanol; drying to obtain raw sugar peptide; and (4) purifying the raw sugar peptide to obtain the abelmoschus esculentus polysaccharide peptide. The method has low production cost; the obtained abelmoschus esculentus polysaccharide peptide has high purity and few three wastes. An anti-saccharifying test shows that the abelmoschus esculentus polysaccharide peptide extracted from abelmoschus esculentus has the anti-saccharifying effect.

Description

technical field [0001] The invention relates to a preparation method of okra polysaccharide peptide, in particular to a method for extracting okra polysaccharide peptide from okra. Background technique [0002] Aging is a process that everyone must go through. Everyone will grow old, but some people age quickly, and some people age slowly. The reason for this is that apart from the excessive oxygen free radicals in the body as the age increases, glycation is also an important factor in aging. Sugar is an essential core nutrient for the human body. The free sugar in the blood will combine with the protein in the body, causing aging and confusion of the skin and body. Importantly, the quality of protein is reduced by binding to sugar. For women, skin aging is the number one killer of beautiful appearance. Under normal circumstances, human skin will appear aging after the age of 30, and it will increase rapidly with age. The number of glycation end products (AGEs) produced by...

Claims

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Application Information

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IPC IPC(8): C07K14/415C07K1/36C07K1/34C07K1/30C07K1/18
CPCC07K14/415
Inventor 张博冯金磊李金楠贾斌朱倩
Owner TIANJIN INT JOINT ACADEMY OF BIOTECH & MEDICINE
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