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A kind of method for extracting total flavonoids of Auranthus nudica

A technology of violet bead and total flavonoids is applied in the directions of pharmaceutical formulations, medical preparations containing active ingredients, plant raw materials, etc., which can solve the problems of many impurities, poor yield, unstable process, etc. Utilization, the effect of short extraction time

Active Publication Date: 2015-11-25
JIANGXI POZIN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the preparation process of the total flavonoids of Adenophora nudica is mainly obtained by extraction with conventional solvents and then refined by macroporous resin, but this method has more impurities, unstable process and poor yield

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] The preparation of embodiment 1 total flavonoids of Auranthus nudica

[0013] Pulverize 100g of Auranthus nudiflora medicinal material, add 30% ethanol aqueous solution, the addition amount of ethanol aqueous solution is 12 times of the weight of Auranthus nudiflora medicinal material, soak for 20 minutes, use microwave to extract, control the radiation power density of microwave to be 15W / g , the irradiation time is 150 seconds, the temperature of the ethanol aqueous solution is controlled below the boiling point, then it is sealed at room temperature and cooled, and the microwave extraction and cooling steps are repeated 4 times. After filtering, the filtrate was concentrated and then heated to dryness to obtain 7.82 g of total flavone products. The ceramic membrane is made of alkali clay, with a bulk density of 1.0 g / cm3, a porosity of 60%, and a pore radius of 2-8 μm.

[0014] Using NaNO 2 -Al(NO 3 ) 3 -NaOH method to measure the total flavonoid content in the o...

Embodiment 2-3

[0016] Except the following table parameter, other methods and parameters are all the same as embodiment 1 of the present invention

[0017]

Embodiment 4

[0019] ICR mice were divided into random groups, 10 in every group. Before the experiment, the mice were fasted for 24 hours and injected with indomethacin intraperitoneally at 0.1ml / 10g. After 0.5h, the administration group was administered by intragastric administration at 0.2ml / 10g respectively. After 3 hours, repeat gavage for one time, and after the second gavage for 2.5 hours, then gavage 50% ethanol at a rate of 0.1ml / 10g. After 1 hour, the mice were sacrificed by cervical spine dissection, and the stomach was dissected and dissolved in formalin solution. The ulcer index was observed and compared, and the maximum long diameter of strip bleeding and the maximum wide diameter perpendicular to the maximum long diameter were measured with a vernier caliper. The product of the two was the ulcer index. 1mm wide is 1 point per mm long; 2mm wide is 2 points per mm long; 3mm wide is 3 points per mm long. Calculate ulcer inhibition rate: inhibition rate=(average model group ulce...

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Abstract

The invention relates to the field of traditional Chinese medicine pharmacy, in particular to a method for extracting total flavonoids of callicarpa nudiflora. The method comprises steps as follows: crushing a medicinal material, namely, the callicarpa nudiflora, adding an ethanol aqueous solution with the additive amount being 12-24 times the weight of the callicarpa nudiflora, soaking for 20-40 min, performing extraction with microwaves, controlling the radiation power density of the microwaves to be in a range of 15-30 W / g and the irradiation time in a range of 150-180 s, controlling the temperature of the ethanol aqueous solution under the boiling point, then performing sealing and cooling at the room temperature, repeating the microwave extraction and cooling steps for 4-6 times, performing ultrafiltration on an extract by a hollow cellulose membrane with the molecular weight cutoff of 2000, filtering a filtrate with a ceramic membrane, and concentrating and then heating the filtrate until the filtrate is dry to obtain a total flavonoid product.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine pharmacy, in particular to a method for extracting total flavonoids from Auranthus nudica. Background technique [0002] Callicarpanudiflora (Callicarpanudiflora Hook. et Arn.) is a plant of the genus Verbenaceae (Verbenaceae). It is mainly produced in Hainan, my country. It is an authentic medicinal material in Hainan, and it is also distributed in Guangdong and Guangxi. Foreign countries are mainly distributed in India, Vietnam and Malaysia. Its roots and leaves can be used as medicine. It has the functions of antibacterial and hemostasis, anti-inflammatory and detoxification, dispelling stasis and swelling, and dispelling wind and dampness. , burns and traumatic bleeding. [0003] Studies have shown that the main components of Auranthus nudica are flavonoids, tannins, terpenes, volatile oils, etc. The total flavonoids can increase the number of platelets, shorten the bleeding and c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/85
CPCA61K36/85A61K2236/333A61K2236/39A61K2236/51
Inventor 肖军平吴永忠陈梁杨伟庆李小锋
Owner JIANGXI POZIN PHARMA
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