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Method of culturing dendrobium officinale plantlets

A technology of Dendrobium officinale and a culturing method, applied in the field of plant tissue culture, can solve the problems of inability to guarantee the survival rate, difficulty in popularization and application, unreasonable medium formula, etc., achieves convenient medium configuration, promotes industrialized production, and is convenient for management effect

Inactive Publication Date: 2015-03-25
ANHUI TONGCHUANG MODERN AGRI INVESTMENT DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the above two applications: the technical scheme disclosed in Chinese Patent Application No. 201310173395.0 is to sow the pollen of mature Dendrobium officinale seeds on the above-mentioned medium, after 3 months the mother seedlings will grow out, and then the seedlings will be artificially inoculated. In actual application, the survival rate cannot be guaranteed, and it is difficult to promote and apply
The technical solution disclosed in Chinese Patent Application No. 201210102034.2 mainly adopts hormone-free tissue culture technology, but the formula of its culture medium is not reasonable and needs further improvement

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A culture medium for Dendrobium candidum tissue culture seedlings of the present embodiment, the medium includes explant induction medium, proliferation subculture medium and rooting medium, wherein: the explant induction medium is used to spread the candidum The seeds in the pods of Dendrobium candidum are induced to germinate and form embryos; the embryo proliferation subculture is used to transfer embryos and cultivate them to obtain seedlings of Dendrobium candidum; the rooting medium is used to transfer to seedlings of Dendrobium candidum and carry out Cultivate to obtain strong seedlings of Dendrobium officinale.

[0031]Specifically in this embodiment, the explant induction medium, the proliferation subculture medium and the rooting medium are specifically as follows: the explant induction medium uses MS medium as the basic medium, and adds 5.5 g of agar in the basic medium / L, sugar 30g / L, activated carbon 0.5g / L, banana 67g / L, self-made organic liquid fertilize...

Embodiment 2

[0046] A kind of Dendrobium officinale tissue culture medium of the present embodiment, its explant induction medium, proliferation subculture medium and rooting medium are specifically as follows: explant induction medium uses MS medium as basic medium, in Add agar 5g / L, white sugar 30g / L, activated carbon 0.5g / L, banana 65g / L, self-made organic liquid fertilizer 1.8g / L, and sterilizer 2ml / L in the basic medium. Proliferation subculture medium is MS medium as basic medium, add agar 5g / L, white sugar 30g / L, activated carbon 0.5g / L, banana 47g / L, sterilant 2ml / L in the basic medium. The rooting medium uses self-made mother liquor as the basic medium, and adds agar 5g / L, white sugar 20g / L, activated carbon 0.5g / L, banana 53g / L, and self-made natural organic liquid fertilizer 1.6g / L in the basic medium.

[0047] The preparation process of explant induction medium, proliferation subculture medium and rooting medium in this example is basically the same as that in Example 1, and wi...

Embodiment 3

[0049]A kind of Dendrobium officinale tissue culture medium of the present embodiment, its explant induction medium, proliferation subculture medium and rooting medium are specifically as follows: explant induction medium uses MS medium as basic medium, in Add agar 9g / L, sugar 28g / L, activated carbon 0.6g / L, banana 75g / L, self-made organic liquid fertilizer 2g / L, and sterilizer 1.5ml / L in the basic medium. MS medium was used as the basic medium for the proliferation subculture medium, and 9 g / L of agar, 32 g / L of sugar, 0.48 g / L of activated carbon, 45 g / L of banana, and 1.5 ml / L of sterilizer were added to the basic medium. The rooting medium uses the self-made mother liquor as the basic medium, and in the basic medium, add agar 9g / L, white sugar 22g / L, activated carbon 0.52g / L, banana 55g / L, self-made natural organic liquid fertilizer 1.5g / L, sterilizer 1.5ml / L.

[0050] The preparation process of explant induction medium, proliferation subculture medium and rooting medium ...

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Abstract

The invention discloses a method of culturing dendrobium officinale plantlets and belongs to the field of plant tissue culturing technology. The method comprises the steps: step 1, induction and culturing of seeds: choosing dendrobium officinale fruit pods as explants, spreading seeds in the fruit pods on an explant induction culture medium and inducing seeds to germinate to form sprouts; step 2, multiplication and subculture of sprouts: grafting the sprouts on a multiplication and subculture medium, placing the multiplication and subculture medium in a culturing room and culturing to obtain dendrobium officinale seedlings; step 3, culturing of rooting seedlings: grafting the dendrobium officinale seedlings on a rooting culture medium and culturing to obtain strong dendrobium officinale seedlings; and step 4, transplanting: hardening the strong dendrobium officinale seedlings subjected to the rooting culturing and then transplanting the strong seedlings. The method of culturing dendrobium officinale plantlets solves problems of slow rooting and lower survival rate in the traditional culturing method of dendrobium officinale and provides convenience to the large-scale culturing of high-quality dendrobium officinale plantlets.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and more specifically relates to a method for culturing tissue culture seedlings of Dendrobium officinale. Background technique [0002] Dendrobium candidum is a perennial herb belonging to epiphytic orchids. It is commonly known as Tiepi Fengdou. Because of its black outer stems, it is also known as black knot grass. Dendrobium is a commonly used precious traditional Chinese medicine with a long history of application. In "Shen Nong's Herbal Classic" It has been listed as top grade, and it has been proven that it has the functions of nourishing yin and clearing heat, promoting body fluid and benefiting stomach, relieving cough and moistening lung, moistening throat and improving eyesight, improving human immune function, preventing tumor and cardiovascular disease, improving sleep, preventing cataract, and prolonging life. However, due to the special growth environment and limited d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 郑毓华于太和周道阳
Owner ANHUI TONGCHUANG MODERN AGRI INVESTMENT DEV
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