Method for making resin slices of mature grain seeds
A production method and technology of resin slicing, applied in the preparation of test samples, sampling devices, etc., can solve the problems of slice curling, easy breakage, and difficult area distribution, and achieve the effect of firm adhesion and simplified steps
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[0066] (1) Drawing materials: remove the mature rice seeds from the ears of rice, peel off the glume carefully with a dissecting needle, take out the complete caryopsis, select 10 normal caryopsis and put them in penicillin bottles for subsequent use;
[0067] (2) Fixed:
[0068] ① Prepare 0.2mol / L phosphate buffer solution (p H 7.4):
[0069] a) Mother liquor A (0.2mol / L disodium hydrogen phosphate aqueous solution): weigh 71.64g Na2HPO4·12H2O, and dilute to 1000mL with distilled water;
[0070] b) Mother liquor B (0.2mol / L sodium dihydrogen phosphate aqueous solution): weigh 31.21g Na H2PO4 2H2O, and dilute to 1000mL with distilled water;
[0071] c) Mix according to the ratio of liquid A: liquid B = 81:19, and adjust the pH to 7.4.
[0072] ② Prepare 10% paraformaldehyde aqueous solution:
[0073] a) Weigh 20g of paraformaldehyde into a 250mL triangular conical flask, add 200mL of distilled water, and put it into a 25*9mm magnetic stirrer;
[0074] c) Prepare 0.1mol / L s...
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