A liquid chromatograph
A liquid chromatograph and chromatographic column technology, which is applied in the field of liquid chromatographs with on-line cleaning function, can solve the problems of taking a long time and being unable to realize highly automated operation of the chromatograph.
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Embodiment 1
[0063] like figure 1 Shown, a kind of liquid chromatograph comprises:
[0064] The first flow channel L3 connected with the injector S5 is used to deliver the first mobile phase S3;
[0065] The second channel L21 is used to transport the second mobile phase S2;
[0066] The first chromatographic column C1 is used for primary separation of the sample;
[0067] The middle chromatographic column C2 is used to capture the substances separated by the first chromatographic column C1;
[0068] Analytical flow path L22, which includes a second chromatographic column C3 and a detector D connected in sequence, for further separation and detection of substances captured in the intermediate chromatographic column C2;
[0069] Waste liquid flow channels L28, L31, L14 are used to discharge waste liquid;
[0070] The cleaning channel L25, the directional switching valve V1 and the multi-channel switching valve V2 used to transport the cleaning solution S1; the first chromatographic colu...
Embodiment 2
[0082] like Figure 8 Shown, a kind of liquid chromatograph, on the basis of the liquid chromatograph described in embodiment 1, also includes:
[0083] The modulating channel IL17 is used to deliver the modulating solution S4; and the modulating channel IL17 is connected to the port e21 of the multi-channel switching valve V2;
[0084] Modulation flow channel IIL18, one end of the modulation flow channel IIL18 is connected to the port d20 of the multi-channel switching valve V2, the other end of the modulation flow channel IIL18 is connected to the first flow channel L3, and connected to the flow channel after the injector S5 .
[0085] Function description: The liquid chromatograph described in embodiment 2, except having all functions of embodiment 1, also has the following special functions:
[0086] 1. The function of cleaning the first chromatographic column with the prepared solution:
[0087] like Figure 9 As shown, turn on the delivery pump IVP4, deliver the pr...
Embodiment 3
[0095] like Figure 13 As shown, a liquid chromatograph, on the basis of the liquid chromatograph described in Embodiment 2, also includes a post switch valve V5, and the post switch valve V5 has an interface m1, an interface n2, an interface x3, an interface y4, interface s5 and interface r6; a filter or a protector B2 is connected between the interface n2 and the interface s5; the interface y4 is connected to a section of the waste liquid flow channel L14, and the interface x3 is connected to the waste liquid flow channel L14 The other section of the first channel L3 is connected; the interface m1 is connected to one section of the first flow channel L3, and the interface r6 is connected to another section of the first flow channel L3.
[0096] Function description: The liquid chromatograph described in embodiment 3, except having all functions of embodiment 1 and 2, also has the following special functions:
[0097] 1. Filter or protector B2 interception function for ins...
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