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Kit for rapidly detecting Ebola virus and application method thereof

A kit and virus technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve problems such as effect and influence, and achieve the effect of simple use and rapid detection

Inactive Publication Date: 2015-02-04
广州万俐英合生物科技有限公司
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  • Abstract
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Problems solved by technology

[0002] Ebola virus (EBOV), the virulent virus that causes Ebola hemorrhagic fever (EBHF) in humans and primates, has been claimed to be transmitted from person to person after studying the genetics of the earliest Ebola case in Sierra Leone More than 300 genetic changes have been identified in , which could affect the effectiveness of diagnostic tests and experimental treatments currently in development

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  • Kit for rapidly detecting Ebola virus and application method thereof

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Embodiment 1

[0024] The present invention provides a kit for rapid detection of Ebola virus. The kit takes Nucleoprotein as the target gene, and designs a one-step reverse transcription plus Real time PCR to amplify the conserved segment of the gene. The probe for this amplified segment uses FAM mark. At the same time, the internal reference GAPDH was introduced, and the DNA fragment of GAPDH was designed and amplified by the ARMS method, and the probe for this amplified fragment was labeled with HEX.

[0025] The kit is prepared by the following steps:

[0026] 1. Design primers and probes for reverse transcription and amplification of Nucleoprotein target genes.

[0027] Design primers for reverse transcription and amplification that can be used simultaneously in the conserved region of the Nucleoprotein gene.

[0028] NP_F:AGAGGAGACAACTGAAGCTAATGC;

[0029] NP_R:CTGATTGCCAAGCTGTTGGA;

[0030] NP-Probe: FAM-CAAGTCTATTCCTTCCGAAATTGGTAGTAG.

[0031] 2. Primer design and probe design f...

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Abstract

The invention discloses a kit for rapidly detecting Ebola virus and aims to provide the kit which is simple in application method and can be used for rapidly detecting the Ebola virus. Technical points are that the kit is prepared by the following steps sequentially: (1) designing a reverse transcription primer, an amplification primer and a probe in a conserved region of a Nucleoprotein gene; (2) designing the reverse transcription primer and the probe which are used simultaneously in the conserved region of a GAPDH gene; (3) preparing an RT-TaqMan kit. The kit belongs to the technical field of gene detection.

Description

technical field [0001] The invention discloses a kit and a using method thereof, in particular to a kit for rapidly detecting Ebola virus and a using method thereof, and belongs to the technical field of gene detection. Background technique [0002] Ebola virus (EBOV), the virulent virus that causes Ebola hemorrhagic fever (EBHF) in humans and primates, has been claimed to be transmitted from person to person after studying the genetics of the earliest Ebola case in Sierra Leone More than 300 genetic changes have been identified in the study, which could affect the effectiveness of diagnostic tests and experimental treatments currently in development. Nucleoprotein (NP) is a nucleoprotein gene of Ebola virus. Contents of the invention [0003] In view of the problems referred to above, the purpose of the present invention is to provide a simple and fast detection kit for Ebola virus. [0004] In order to solve the problems of the technologies described above, the first t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68
CPCC12Q1/70C12Q1/701C12Q2600/112C12Q2600/158C12Q2561/113
Inventor 覃启红温华杰黄龙
Owner 广州万俐英合生物科技有限公司
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