SS-COI (Sirex noctilio F. specific cytochrome oxidase I) primer pair and quick molecular detection method
A technology of molecular detection and specificity, applied in biochemical equipment and methods, measurement/testing of microorganisms, DNA/RNA fragments, etc., can solve the problems of no identification characteristics, laborious, time-consuming, etc., and achieve low professional technical ability requirements, The fast and stable effect of the identification method
Inactive Publication Date: 2014-12-24
BEIJING FORESTRY UNIVERSITY
View PDF0 Cites 9 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
In order to distinguish the pine wasp from the closely related tree wasp insects, extensive morphological identification work is required, most of which are time-consuming, labor-intensive and require
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Login to View More
PUM
Login to View More
Abstract
The invention provides an SS-COI (Sirex noctilio F. specific cytochrome oxidase I) primer pair and a quick molecular detection method. By taking Sirex noctilio F. as a target and other four horntails (Sirex nitidus, Sirex nitobei, Tremex fuscicornis and Tremex apicalis) as references, species specific primers based on mitochondrial COI gene sequences are designed and a quick molecular detection system of the Sirex noctilio F. is constructed and optimized, wherein the Sirex noctilio F. and the other four horntails occur in the same region in China and belong to sibling species.
Description
technical field [0001] The invention relates to the field of molecular detection, in particular to a pair of pine wasp-specific SS-CO I primers and a rapid molecular detection method. Background technique [0002] With the acceleration of global economic integration, the situation of alien species invasion in forestry is becoming increasingly severe. Some major forestry invasive species have caused great losses to my country's forest resources, species diversity and ecological security. [0003] The pine wasp (Sirex noctilio F.) is native to Eurasia and North Africa, and mainly harms pine trees. Listed as a "very high risk" forestry invasive species in the United States. In the last century, the insect successively invaded New Zealand and Australia in Australia, Uruguay, Argentina, Brazil and Chile in South America, and South Africa; at the beginning of this century, it was introduced into Canada and the United States in North America; in severely invaded areas, it can cau...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More
IPC IPC(8): C12N15/11C12Q1/68
Inventor 陶静骆有庆任利利石娟
Owner BEIJING FORESTRY UNIVERSITY
Who we serve
- R&D Engineer
- R&D Manager
- IP Professional
Why Patsnap Eureka
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com