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Artificial breeding method for wild lampetra morii

A technology of artificial breeding and lamprey, which is applied in fish farming, application, climate change adaptation, etc., to achieve the effects of large-scale farming, large economic application value, and good breeding effect

Active Publication Date: 2014-11-12
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to overcome the defects and deficiencies of the existing lamprey artificial breeding technology, and provide a method for artificial breeding of wild lamprey, thereby creating conditions for laboratory and large-scale breeding of northeast lamprey

Method used

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  • Artificial breeding method for wild lampetra morii

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The artificial breeding of embodiment 1 wild northeast lamprey

[0031] 1. Select the breeding place of the Northeast Lamprey, which is the Shanding Lake in the waters of the Yalu River on the border between China and North Korea. During the breeding season of the Northeast Lamprey, put multiple grille fish cages into the depths of the water, lift the cages after 3 days, and obtain more than 100 fish cages. Vigorous sexually mature males and females.

[0032] 2. Use ice cubes, fish water, and air in a volume ratio of 1:2:2 to transport them to the artificial breeding site. The transportation time on the way shall not exceed 24 hours to prevent the lamprey from dying due to lack of oxygen or excessive temperature. The transported lampreys were reared in a constant temperature breeding box at 15°C with a photoperiod of 12h:12h. Because Northeastern lampreys do not eat when they are sexually mature, they only need to change fresh water twice a week.

[0033] 3. Artifi...

Embodiment 2

[0037] Embodiment 2 Artificial breeding of wild northeast lamprey

[0038] 1. Select the breeding place of the Northeast Lamprey, which is the Shanding Lake in the waters of the Yalu River on the border between China and North Korea. During the breeding season of the Northeast Lamprey, put multiple grille fish cages into the depths of the water, lift the cages after 3 days, and obtain more than 100 fish cages. Vigorous sexually mature males and females.

[0039] 2. Use ice cubes, fish water, and air in a volume ratio of 1:2:2 to transport them to the artificial breeding site. The transportation time on the way shall not exceed 24 hours to prevent the lamprey from dying due to lack of oxygen or excessive temperature. The transported lampreys were reared in a constant temperature breeding box at 15°C with a photoperiod of 12h:12h. Because Northeastern lampreys do not eat when they are sexually mature, they only need to change fresh water twice a week.

[0040]3. Artificial ...

Embodiment 3

[0044] Embodiment 3 Artificial breeding of wild northeast lamprey

[0045] 1. Select the breeding place of the Northeast Lamprey, which is the Shanding Lake in the waters of the Yalu River on the border between China and North Korea. During the breeding season of the Northeast Lamprey, put multiple grille fish cages into the depths of the water, lift the cages after 3 days, and obtain more than 100 fish cages. Vigorous sexually mature males and females.

[0046] 2. Use ice cubes, fish water, and air in a volume ratio of 1:2:2 to transport them to the artificial breeding site. The transportation time on the way shall not exceed 24 hours to prevent the lamprey from dying due to lack of oxygen or excessive temperature. The transported lampreys were reared in a constant temperature breeding box at 15°C with a photoperiod of 12h:12h. Because Northeastern lampreys do not eat when they are sexually mature, they only need to change fresh water twice a week.

[0047] 3. Artificial...

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Abstract

The invention provides an artificial breeding method for wild lampetra morii. The method comprises the following steps: capturing sexually mature parent lampreys with high activity; gently massaging and extruding the abdomen of the female parent lamprey, squeezing egg cells into a clean fertilization container, squeezing seminal fluid of the male parent lamprey on the egg cells, injecting sterile water, slightly and uniformly shaking, and standing at the temperature of 15-21 DEG C for performing artificial insemination; slightly cleaning excessive seminal fluid on the egg cells by using the sterile water; incubating the oosperm in an incubator at the temperature of 15-21 DEG C, separating the incubated lamprey juvenile fish in a constant temperature breeding box of which the bottom is paved with fine sand and which has a circulating water flow system at the temperature of 15-21 DEG C, breeding for 20-25 days, feeding yeasts in an amount of 50-100ug per fish, and feeding once a day until the wild lampetra morii fry is obtained. According to the method, the technical problem that the wild lampetra morii is difficult to breed in an artificial manner is solved, and conditions are created for large-scale lamprey breeding in labs.

Description

technical field [0001] The invention belongs to the technical field of breeding of jawless fishes. More specifically, it relates to captive breeding methods for wild Northeast lampreys. Background technique [0002] Lamprey ( Lampetra japonicum ), also known as hagfish, seven stars, and small brittle bones, is an ancient fish of the round mouth class and the order of lampreys. In China, it is mainly distributed in the waters of the Yalu River. It is a land-sealed type rather than a migratory type. It is called the Northeast Lamprey. Lamprey is one of the few jawless vertebrate fish-like animals that have survived so far. Known as a "living fossil", it is a model animal for the study of vertebrates and is of great significance for the study of biological evolution. [0003] At the same time, lamprey is also a valuable aquatic product with high economic and social benefits, especially as a special product in Northeast China, which has attracted our attention. Europe has r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 吴芬芳张涛涛徐安龙
Owner SUN YAT SEN UNIV
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