Blueberry tissue cultured seedling liquid rooting method and special culturing container

Abstract

The invention discloses a blueberry tissue cultured seedling liquid rooting method and a special culturing container. The rooting method includes the following steps that a solution is prepared, a liquid medium containing certain kinds of hormones with certain concentration is prepared and dispensed in outer boxes in a tip box, a support is placed, and after package is performed with an edible fungi bag, autoclaved, inoculated and cultured for rooting are carried out; the special culturing container is formed by transforming the tip box and comprises the tip box and the support in the tip box, a plurality of support holes are formed in the support, and the height, the number of the holes and the hole diameter of the support are adaptive to the length and the thickness of young sprouts newly cultured. The blueberry tissue cultured seedling liquid rooting method has the advantages that the formula of the medium is optimized, and the support is placed in the tip box, so that the rooting rate in blueberry tissue cultured seedling bottles is increased substantially, the root system quality indexes such as the rooting number of the young sprouts newly cultured and the length of roots are increased, the problem that a liquid rooting technology is lack of an effective fixed supporting object is solved, and suppression on tissue cultured seedlings by a solid medium agar coagulator is avoided.

Description

technical field [0001] The invention relates to a method for promoting rooting of plants, in particular to a method for promoting rooting of blueberry tissue culture seedlings by using a liquid medium, and belongs to the technical field of plant cultivation. Background technique [0002] Blueberry (blueberry), also known as lingonberry, is a perennial shrub of the Rhododendronaceae Vaccinium genus. It is a new tree species with high economic value and broad development prospects. The fruit is blue, the size of the fruit varies with the variety, and the weight of a single fruit is 0.5-2.5 grams. The pulp is delicate, the seeds are small, sweet and sour, and the flavor is unique. It is one of the representative varieties of the fastest growing third-generation fruit trees in the world today. At the same time, blueberry fruit is rich in nutrition and can be eaten fresh directly, and has strong medicinal value and nutritional and health functions. [0003] Plant tissue culture...

AI Technical Summary

Problems solved by technology

However, these agar substitutes have one or more of the problems of easy contamination of explants, difficult inoculation, easy injury, ineffective fixation, and incapable of factory application.

Images