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Wheat 4B chromosome grain weight QGW4B-CAPS molecular marker and application thereof

A technology of QGW4B-CAPS1 and QGW4B-CAPS2, applied in the field of wheat 4B chromosome grain weight QGW4B-CAPS molecular markers, can solve the problems of poor accuracy, reduce the actual efficiency of molecular assisted selection, too many QTLs, etc., and achieve the goal of accurate breeding Effect

Active Publication Date: 2014-10-08
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the main problems of molecular marker-assisted selection of wheat grain weight are: most of the QTLs are not functional markers, and most of the published QTLs have not been verified through the breeding process or variety; Gene loci, the accuracy is poor, and due to the large confidence interval, too many QTLs or false positive QTLs, the actual efficiency of molecular assisted selection is reduced, and it cannot even be effectively applied to wheat breeding

Method used

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  • Wheat 4B chromosome grain weight QGW4B-CAPS molecular marker and application thereof
  • Wheat 4B chromosome grain weight QGW4B-CAPS molecular marker and application thereof
  • Wheat 4B chromosome grain weight QGW4B-CAPS molecular marker and application thereof

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Experimental program
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Effect test

Embodiment 1

[0051] The grain weight control site QGW4B-17 of wheat 4B chromosome is located at 31.3-36.3 cM of the short arm of wheat 4B chromosome, between the molecular markers Ex_c101685_705 and RAC875_c27536_611 of the wheat 4B chromosome.

[0052] The RIL population constructed by the genetic map construction and QTL mapping laboratory was used as the material: the RIL population was crossed with the common wheat line Shannong 01-35 as the female parent and Gaocheng 9411 as the male parent to obtain the F1 generation. After 9 generations, a RIL with 173 pedigrees (F 9：10 )group.

[0053] The parent Shannong 01-35 is a large-grain, medium-gluten wheat line with low ear formation rate bred by Shandong Agricultural University, and it is a common commercial product;

[0054] The male parent, Gaocheng 9411, is a strong gluten wheat line with small grains and high tillering rate, which was bred by the Gaocheng Agricultural Science Institute of Hebei Province. It is a common commercial pro...

Embodiment 2

[0066] The application of the molecular marker developed according to the grain weight control site QGW4B-17 of the wheat 4B chromosome described in Example 1 in molecular assisted breeding is to use the molecular marker closely linked with the major QTL of 1000-grain weight in wheat for the detection of wheat varieties or A method of strain, which includes the following steps:

[0067] 1. Molecular markers are synthesized in biotechnology companies such as BGI, and the sequence is as follows:

[0068] QGW4B-CAPS1: Upstream primer: 5'-TAATAGGCTTGAAAAGTTCTTAC-3', SEQ ID NO. 1

[0069] Downstream primer: 5'-CGAATAGACTTCAGGGGCA-3'; SEQ ID NO.2

[0070] QGW4B-CAPS2: Upstream primer: 5'-CCCGGTTTTATGAACGATGAGTGG-3'; SEQ ID NO. 3

[0071] Downstream primer: 5'-GCGTGACAATTGCCACAGATCTA-3. SEQ ID NO.4

[0072] 2. Extract the DNA of the detection material: refer to the plant DNA extraction method provided by Triticarte Pty. Ltd (http: / / www.triticarte.com.au)...

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Abstract

The invention relates to a wheat 4B chromosome grain weight QGW4B-CAPS molecular marker and an application thereof. A grain weight control genetic locus QGW4B-17 is positioned at 31.3-36.3cM of a short arm of a wheat 4B chromosome and is positioned between a wheat 4B chromosome molecular marker Ex-c101685-705 and a molecular marker RAC875-c27536-611. The invention also discloses a grain weight QGW4B-CAPS molecular marker developed according to the grain weight control genetic locus QGW4B-17. According to the molecular marker QGW4B-CAPS, large grain resources containing the gene feature base sequence can be selected in a purposeful mode and are used for high-yield wheat breeding or molecular designing convergent cross breeding, and molecular marker assisted selective breeding is performed by using the marker, so that breeding of high-yield wheat variety is rapidly and accurately performed.

Description

technical field [0001] The invention relates to a wheat 4B chromosome grain weight QGW4B-CAPS molecular marker and its application, belonging to the field of biotechnology. Background technique [0002] Wheat is one of the three major food crops in the world and the main ration of the population in northern my country. With the reduction of per capita arable land and freshwater resources in my country, the problem of food supply security has become increasingly prominent. Thousand-kernel weight is one of the three elements of wheat yield, and increasing wheat kernel weight is an important way to cultivate high-yield wheat. [0003] Grain weight is a quantitative trait jointly controlled by major genes and minor genes. The number of genes is large and the effect is low, and it is easily affected by the environment. In traditional breeding, large-scale field selection is required, which is labor-intensive and inefficient. Wheat is allohexaploid, the genes related to grain w...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 陈建省田纪春李青芳刘凯陈广凤谷植群
Owner SHANDONG AGRICULTURAL UNIVERSITY
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