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Microbial preparation for brackish water aquaculture, and preparation method and application thereof

A technology of microbial preparations and brackish water, applied in chemical instruments and methods, biological water/sewage treatment, water/sludge/sewage treatment, etc. To achieve good water quality environment, promote reproduction and growth, and reduce the incidence of diseases

Inactive Publication Date: 2014-09-10
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because nitrite oxidizing bacteria also have the characteristics of autotrophy, aerobicity, and dependence, the generation time is long, the growth is slow, and the natural number in the water body is limited, resulting in poor nitrification. In the process of purifying water bodies Can't have the desired effect

Method used

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  • Microbial preparation for brackish water aquaculture, and preparation method and application thereof
  • Microbial preparation for brackish water aquaculture, and preparation method and application thereof
  • Microbial preparation for brackish water aquaculture, and preparation method and application thereof

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preparation example Construction

[0026] A microbial preparation for brackish water culture and a preparation method thereof, the method steps are as follows:

[0027] The first step is to produce seeds;

[0028] In the second step, the saccharomycetes and Acinetobacter in step 1 are separately cultured to obtain a bacterial liquid, and Bacillus subtilis and Bacillus megaterium are compounded together to obtain a bacterial liquid;

[0029] Wherein, the components of the culture medium for Bacillus subtilis and Bacillus megaterium are: 20g of glucose, 15g of peptone, 2-10g of sodium chloride, 0.5g of beef extract, 1L of distilled water, and pH 7.0.

[0030] Wherein, the yeast culture medium components are: 200g of potatoes (to prepare potato juice), 20g of glucose, 2-10g of sodium chloride, 1L of distilled water, pH 7.0.

[0031] Wherein, the Acinetobacter culture medium components are: sodium acetate 0.5g, sodium nitrate 0.36g, disodium hydrogen phosphate 0.5g, potassium dihydrogen phosphate 0.5g, magnesium s...

Embodiment 1

[0038] A microbial preparation for brackish water culture and a preparation method thereof, comprising the following steps:

[0039] The first step is to produce seeds;

[0040] In the second step, the yeast and Acinetobacter obtained in the first step of seed production are separately cultured to obtain a bacterial liquid, and Bacillus subtilis and Bacillus megaterium are co-cultured together to obtain a bacterial liquid, cultivated at 25° C., and cultivated for 36 hours;

[0041] In the third step, 20 g of Bacillus subtilis, 20 g of Bacillus megaterium powder, 25 g of saccharomyces, 30 g of Acinetobacter and 100 g of zeolite powder were respectively weighed according to the weight of the bacterial liquid, and the mixture was dried, pulverized, and sieved to obtain a microbial preparation.

[0042] A kind of application of microbiological preparation for brackish water culture, the microbiological preparation that above-mentioned method is made is used for vannamei culture, a...

Embodiment 2

[0044] A microbial preparation for brackish water culture and a preparation method thereof, comprising the following steps:

[0045] The first step is to produce seeds;

[0046] In the second step, the yeast and Acinetobacter obtained in the first step of seed production are separately cultured to obtain a bacterial liquid, and Bacillus subtilis and Bacillus megaterium are jointly cultured to obtain a bacterial liquid, cultivated at 35° C., and cultivated for 20 hours;

[0047] In the third step, 25 g of Bacillus subtilis, 10 g of Bacillus megaterium powder, 20 g of saccharomyces, 35 g of Acinetobacter and 200 g of zeolite powder were respectively weighed according to the weight of the bacterial liquid, and the mixture was dried, pulverized, and sieved to obtain a microbial preparation.

[0048] A kind of application of microbiological preparation for brackish water culture, the microbiological preparation that above-mentioned method is made is used for vannamei culture, and the...

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Abstract

The invention relates to a microbial preparation for brackish water aquaculture, which comprises the following components in parts by weight: 20-25 parts of Bacillus subtilis, 10-20 parts of Bacillus megaterium, 20-25 parts of microzyme, 30-35 parts of Acinetobacter and 100-200 parts of carrier. The preparation method comprises the following steps: sequentially inoculating Bacillus subtilis, Bacillus megaterium, microzyme and Acinetobacter seed culture solutions into a culture medium, fermenting, and mixing the obtained bacterium solution with the carrier substance to obtain the microbial preparation. The microbial preparation is applicable to a culture water body with the salinity range of 0.2-1%. The microbial preparation can obviously improve the water quality of the brackish water aquaculture water body, especially obviously lowers the nitrite content in the water body, and can enhance the flesh texture of the cultured animals.

Description

technical field [0001] The invention belongs to the technical field of microbial preparations, in particular to a microbial preparation for aquaculture in brackish water, a preparation method and application. Background technique [0002] Nitrifying bacteria (Nitrite-oxidizing bacteria, NOB) is a large group of microbial flora that play a key role in the natural nitrogen cycle, but due to their special ecological and physiological properties, they grow slowly, with an average generation time of about 10 hours, and are susceptible to The influence of the external environment restricts its application. At present, the problem of nitrogen pollution in brackish water aquaculture in China is becoming more and more serious, and it is urgent to control nitrogen pollution. [0003] Due to the rapid development of my country's brackish water aquaculture industry and the continuous increase in aquaculture density, the ecological environment in the aquaculture water body has been destr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34
Inventor 袁春营王婉莹杨硕崔青曼
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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