Transgenic plant cultivation risk evaluation method based on soil fauna removal

A technology of transgenic plants and soil animals, applied in the fields of botanical equipment and methods, horticultural methods, horticulture, etc., can solve the problem of not being able to separate and distinguish the response of specific sensitive groups to the planting of transgenic plants, and not truly reflecting the state of the field soil.

Inactive Publication Date: 2014-09-10
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The former method is a field investigation, and the problem is that all animals are gathered together, and the response of specific sensitive groups to transgenic plant planting cannot be separated; the latter method can independently evaluate the impact of transgenic plant planting on specific soil sensitive groups , but the disadvantage is that the experimental conditions are culture medium, which does not truly reflect the state of the soil in the field, and thus truly reflect the natural soil environment where the transgenic plants are planted.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1) To fauna: set 0.1m 3 (Length × width × height = 1m × 1m × 0.1m) After the in-situ soil of the transgenic plant planting area is taken out from the farmland, pass through a 2mm sieve to remove large soil clods, and then carry out high-temperature (110°C, 30 minutes) sterilization, The whole biota was removed, and the obtained fauna-removed soil was subpackaged into 10 transparent glass containers (15 cm high, 10 cm in diameter), and the soil was filled to a depth of 10 cm.

[0035] 2) Preparation of soil filtrate and addition of soil filtrate: Use 100g of in-situ rhizosphere soil, add 500ml of distilled water, stir well, let it stand for 24 hours, filter with 1mm, 180μm, 75μm, 45μm, 45μm and 20μm aperture screens successively, filter out For soil fauna adults and eggs, add 20ml of the prepared soil filtrate to the container containing the soil from which the soil fauna has been removed, to restore the microflora.

[0036] 3) Plant planting: seeds of transgenic corn B...

Embodiment 2

[0044] 1) Fauna removal: After the in-situ soil is taken out from the farmland, it is passed through a 2mm sieve to remove large soil clods, and then sterilized at high temperature (110°C, 30 minutes) to remove the entire biota to obtain Fauna soil.

[0045] 2) Preparation and addition of soil filtrate: Use 100g of in-situ rhizosphere soil, add 500ml of distilled water, stir well and let it stand for 24 hours, then use 1mm, 180μm, 75μm, 45μm, 45μm and 20μm sieves to filter out the soil For animal adults and eggs, add 20ml of the prepared soil filtrate to the in-situ soil where the soil fauna has been removed, and restore the microflora.

[0046] 3) Planting of crops: the seeds of transgenic maize NC6304YGRR and control variety NC6304 were planted in the soil obtained in step 2), and 5 replicates were set for each variety.

[0047]4) According to the future climate change control conditions: put the container after planting in step 3) in the climate chamber, and according to t...

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Abstract

The invention provides a transgenic plant cultivation risk evaluation method based on soil fauna removal. The method mainly comprises the steps of fauna removal, soil filter liquor adding, plant cultivation, experimental fauna adding, sampling analysis and the like. Soil fauna adults and spawns are killed and removed through high-temperature sterilization, farmland in-situ microorganisms are restored through soil filter liquor, meanwhile, laboratory standardization soil fauna feeding is combined, and the influence of transgenic plant cultivation on the survival rate and the single-generation and multi-generation breeding potential of the single soil faunas is evaluated in close-to-nature in-situ soil. The defects that originally-adopted field investigation cannot effectively control environmental factors, and the field soil environment cannot be fully reflected by indoor culture dish breeding are overcome. The method has the dual advantages of transgenic plant cultivation in-situ evaluation and the specific intolerant group study, and a method system for evaluating the influence of transgenic plant cultivation on a single sensitive soil fauna group in the close-to-nature state is comprehensively built.

Description

technical field [0001] The invention belongs to the field of agricultural production, and in particular relates to an ecological risk assessment method for plants obtained through genetic engineering. Background technique [0002] Soil animals refer to animals that spend a long period of time or in a certain stage of their life history in the soil or surface litter layer. Soil animals play an important role in the decomposition of litter, the improvement of soil fertility, and the material circulation and energy flow of underground ecosystems. Previous studies on the impact of transgenic plants on soil non-target animals, on the one hand, focused on the in situ survey of the sample plot, that is, in the field of transgenic plants and control varieties, in situ sampling and separation of soil animals were used to evaluate the impact of transgenic plants on soil animal populations. On the other hand, it is mainly to use the medium and feed indoors to study the impact of trans...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01G23/00A01G7/00
Inventor 常亮宋新元王柏凤吴东辉刘金文武奉慈刘娜龙丽坤王大铭
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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