Allium cepa cytoplasmic male sterile SCAR marker and application thereof

Abstract

The invention discloses an Allium cepa cytoplasmic male sterile SCAR marker. Specifically, the specific fragment of the SCAR marker is 2175bp long, and the nucleotide sequence is shown as SEQ ID No.2; a common fragment is 1053bp long, and the nucleotide sequence is shown as SEQ ID No.3. The invention also discloses primers of the SCAR marker, and the primers include a forward primer and a reverse primer respectively shown as SEQ ID No.4 and SEQ ID No.5. The cytoplasmic type of the Allium cepa material can be rapidly and accurately judged by the SCAR molecular marker and the primers involved in the invention, misclassification caused by experimental misoperation and invalid amplification of PCR can be avoided, breeding of Allium cepa cytoplasmic male sterile new lines can be accelerated, and a novel Allium cepa hybrid parent selection system is established.

Description

technical field [0001] The invention relates to a SCAR marker and an application thereof, in particular to an onion cytoplasmic male sterility SCAR marker and its application in identifying onion cytoplasmic genotypes, and belongs to the field of biotechnology. Background technique [0002] Onion (Alliumcepa.L), also known as onion, onion, Liliaceae, Allium, biennial plant, has a cultivation history of more than 5,000 years, and is one of the main vegetable species in the world. Cultivation, storage and processing of onions in China. In: the international symposyium on the utilization and processing of onions, 1997, 11:107-121]. According to preliminary statistics, my country's onion planting area reached 226,000 hm 2 (1999), ranking second in the world, with a total output of 4.629 million t, ranking first in the world [Su Baole. Foreign Exchange Earning Vegetable Export Guide. Beijing: China Agricultural Science and Technology Press, 1999:180-195]. In 2000, the onion out...

AI Technical Summary

Problems solved by technology

Theoretically speaking, the SCAR marker can distinguish onion S-type and N-type cytoplasm through a single PCR, but in practical application, it may eventually lead to wrong cytoplasm type classification due to experimental operation errors and invalid amplification of PCR. The application of the results in field breeding will bring irreparable losses to the breeding work

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