Experimental method for measuring indoor control effect on soybean phytophthora root rot caused by chemical agents

Abstract

The invention relates to an experimental method for measuring indoor control effect on soybean phytophthora root rot caused by chemical agents. Soybean phytophthora root rot is of foreign quarantine pests of China and belongs to typical soil-borne disease. Literature reports that the soybean phytophthora root rot develops by 3 times each year and threatens soybean production of China. At present, an irrigation bacterial liquid bacterial inoculation method, a hypocotyledonary axis wound inoculation method, a hypocotyledonary axis zoospore inoculation method and a bacterial layer inoculation method are generally adopted for measuring potted living bodies with the soybean phytophthora root rot by the chemical agent in a lab, but the four experimental methods have certain defects, for example, the concentration of obtained zoospore suspensions cannot be kept consistent; the inoculation speed is low, the workload is high, and the death of a large quantity of diseased plants is easily caused; zoospores generated by different phytophthora sojae plants are different in number and size; the germination rate of soybean seeds is low and the rot, dead seedlings and the like of the soybean seeds are easily caused. The experimental method adopted by the invention is simple to operate, soybeans can be uniformly infected with phytophthora sojae, the control morbidity is uniform and stable and is kept at 85%-95%, the difference between a chemical agent applying region and a control region can be well distinguished, and the symptoms of disease can be simply and quickly observed.

Description

technical field [0001] The invention belongs to the field of disease prevention and control test methods, in particular to a test method for measuring the indoor control effect of medicaments on soybean Phytophthora root rot. Background technique [0002] Soybean Phytophthora Root Rot is a typical soil-borne disease caused by Phytophthora soybean infestation. It is one of the devastating diseases in soybean production and causes extremely serious losses. Susceptible varieties can cause losses of more than 25% to 50%, and the protein content of the damaged seeds is significantly reduced. The disease is a first-class pest in my country's foreign quarantine, and has also been included in the list of national agricultural plant quarantine pests. [0003] The pathogen can cause harm at all stages of soybean growth, including rotten seeds before emergence of seedlings, rotten seedlings and sudden death of seedlings after emergence, causing seedlings to break ridges and infestatio...

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Problems solved by technology

[0030] The advantage of this method is that the inoculation disease conditions are very close to the natural disease conditions, the identification period is short, and the consistency of the experimental