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Liquid moving gun inserting device for biologic molecule purification and separation

A technology of biomolecules and pipetting, which is applied in biochemical cleaning devices, enzymology/microbiology devices, bioreactors/fermenters for specific purposes, etc. The effect of skewed gun head and good sealing

Active Publication Date: 2014-07-02
苏州佰然基因科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a pipette tip insertion device for the purification and separation of biomolecules to solve the problems of skewed pipette tips and poor sealing in the existing pipetting device during the replacement and installation of pipette tips

Method used

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  • Liquid moving gun inserting device for biologic molecule purification and separation
  • Liquid moving gun inserting device for biologic molecule purification and separation

Examples

Experimental program
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Effect test

Embodiment Construction

[0023] The specific implementation manners of the present invention will be further described in detail below in conjunction with the accompanying drawings and examples. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0024] Such as figure 1 As shown, the pipette tip insertion device for the purification and separation of biomolecules provided by the present invention includes an installation frame 1, eight hollow tubes 2, a stopper 3, a spring 4 and a sealing rubber ring 5, and the installation frame 1 is installed On the slider mechanism, the installation frame 1 can move horizontally and vertically under the action of the slider mechanism. The lower frame of the installation frame 1 is provided with eight through holes arranged side by side, and the upper frame of the installation frame 1 is provided with The groove corresponding to the hole of the lower frame, the hollow tube 2 passes...

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PUM

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Abstract

The invention relates to the technical field of biologic molecule purification and separation, and in particular relates to a liquid moving gun inserting device for biologic molecule purification and separation. The liquid moving gun inserting device comprises a mounting frame, a hollow tube, a baffle and an elastic buffer piece, wherein the hollow tube penetrates through the lower frame side of the mounting frame; the hollow tube is positioned in the frame by virtue of the baffle mounted on the hollow tube; the elastic buffer piece is arranged between the upper frame side of the mounting frame and the baffle; in the process of inserting a to-be-mounted gun, under the action of a sliding block mechanism, the mounting frame moves downwards, when the lower end of the hollow tube is contacted with a gun head, the hollow tube and the mounting frame perform certain relative movement, and because of the compression force generated when the elastic buffer piece is compressed, the lower end of the hollow tube is abutted with the gun head in a buffered manner, so that the gun head can be stably and reliably inserted and taken out from the lower end of the hollow tube, furthermore the inserted gun head is prevented from tilting, and moreover good sealing of the lower end of the hollow tube and the gun head is achieved.

Description

technical field [0001] The invention relates to the technical field of purification and separation of biomolecules, in particular to a pipette tip insertion device for purification and separation of biomolecules. Background technique [0002] At present, the nucleotide extraction and purification methods for biomolecules mainly include: (1) Lysis: prepare a centrifuge tube containing a lysate, place the cells to be operated in the centrifuge tube for lysis, and rupture the cells to release DNA; ( 2) Binding: Use magnetic beads, etc. as the solid-phase carrier, and place the solid-phase carrier in a centrifuge tube to combine the released DNA with the solid-phase carrier; (3) Cleaning: Put a sheathed magnetic rack (such as a magnetic rod, etc.) into the centrifuge tube to absorb the magnetic beads, then draw out the binding solution with a hand-held pipetting device, then wash the magnetic beads, and then draw out the cleaning solution; (4) Elution: inject the eluent into the...

Claims

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Application Information

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IPC IPC(8): C12M1/00
CPCB01L9/543B01L2300/0861
Inventor 聂棱
Owner 苏州佰然基因科技有限公司
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