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Preparation method of metal chelating protein hydrolytic peptide

A metal chelate peptide and composite protease technology, applied in the biological field, can solve the problems of zinc absorption and metabolism limitation, zinc deficiency, etc.

Active Publication Date: 2014-06-25
FUZHOU UNIV
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Problems solved by technology

[0005] Although zinc sources are very abundant in nature, the absorption and metabolism of zinc in the human body is restricted by various factors. Zinc deficiency has become a public health problem in my country and many developing countries.

Method used

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  • Preparation method of metal chelating protein hydrolytic peptide
  • Preparation method of metal chelating protein hydrolytic peptide

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Embodiment 1

[0017] The preparation method is as follows:

[0018] The whey protein (WPC 80) used in this technique was purchased from HILMAR (Texas, USA), and the enzyme was purchased from Novozymes Biotechnology Co., Ltd. (Tianjin, China). Single factor experiments were used to investigate four enzymatic hydrolysis factors, namely substrate concentration (1%, 3%, 5% and 7%), enzymatic hydrolysis temperature (40, 50, 55 and 60 ℃), enzyme Mixing ratio (compound protease: flavor protease = 1:1, 1:2, 1:3 and 2:1 w / w), enzyme-substrate ratio (1:100, 1:50, 1:25 and 1 :20 w / w) and digestion time (1, 3, 5, 7, 9 hours).

[0019] Weigh 5.0 g of whey protein and dissolve it in 100 ml of distilled water, then adjust its pH to 7.0 with 2 mol / L NaOH. First heat the solution to 49°C in a water bath, then add the corresponding amount of enzyme according to the enzyme-substrate ratio of 1:25, the mass ratio of compound protease to flavor protease is 2:1, and the enzymatic hydrolysis time is 7 hours . ...

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Abstract

The invention provides a metal (Ca, Fe, Zn) chelating peptide prepared by enzymolysis with compound protein enzyme and flavor proteinase. Whey protein used as the raw material is subjected to enzymolysis by compound protein enzyme and flavor proteinase, and separation and purification are performed to obtain the purified specific metal (Ca, Fe, Zn) chelating peptide of which the amino acid complete sequence is gy. The metal chelating peptide can be used for producing a novel metal replenisher-peptide chelated calcium, peptide chelated iron or peptide chelated zinc. The novel metal replenisher has a unique chelation system and operation mechanism, can be easily absorbed, has the advantages of high safety, no toxicity and low price, can simultaneously replenish amino acids and metal ions, and can become the first choice of the calcium, iron or zinc replenisher. The invention provides a new idea for application of the whey protein.

Description

technical field [0001] The invention relates to a metal (Ca, Fe, Zn) chelating peptide, more specifically to a metal chelating peptide prepared by hydrolyzing whey protein with complex protease and flavor protease, and belongs to the field of biotechnology. Background technique [0002] With the rise of milk-derived active peptide research and product development, it has been found that whey protein contains potential peptides with biological activities such as immune regulation, lowering blood pressure, lowering blood sugar, lowering cholesterol, antioxidant, antibacterial and antiviral. Whey protein-derived bioactive peptides have extremely high nutritional value, food safety and physiological health functions, and play an important role in promoting the growth, development and disease prevention of the body. They can be used as functional foods or food additives or even Drugs are applied to people's daily life. [0003] At present, calcium deficiency is a global nutritio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K5/062C07K1/20C07K1/18C07K1/16C12P21/06
Inventor 汪少芸黄顺丽赵立娜蔡茜茜邵彪方卫东
Owner FUZHOU UNIV
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