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4-mercaptophenylboronic acid immobilized graphene oxide composite nanometer material, preparation and application thereof

A technology of composite nanomaterials, mercaptophenylboronic acid, applied in peptide preparation methods, chemical instruments and methods, organic chemistry, etc., can solve problems such as not easy to dissolve, weak interaction, and difficult research and application of graphene

Active Publication Date: 2014-06-25
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

Graphene with a complete structure is a two-dimensional crystal composed of six-membered benzene rings without any unstable bonds. It has high chemical stability, its surface is in an inert state, and its interaction with other media is weak. There is a strong van der Waals force between the sheet and the sheet, which is easy to aggregate and makes it difficult to dissolve, which has caused great difficulties for the further research and application of graphene.

Method used

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  • 4-mercaptophenylboronic acid immobilized graphene oxide composite nanometer material, preparation and application thereof
  • 4-mercaptophenylboronic acid immobilized graphene oxide composite nanometer material, preparation and application thereof
  • 4-mercaptophenylboronic acid immobilized graphene oxide composite nanometer material, preparation and application thereof

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Embodiment Construction

[0024] 1. Preparation of composite nanomaterials of GO / PEI / Au / MPB: according to figure 1 Therefore, GO / PEI was prepared by utilizing the electrostatic interaction between graphene oxide and polyethyleneimine. Weigh 0.1 mg of GO, add 1 mL of deionized water, and sonicate for 3 hours to completely peel off GO to form a uniform solution, then add 15 mg of PEI (Mn600), and stir vigorously for 30 minutes after sonicating for 30 minutes to prepare uniform GO / PEI. The unreacted PEI was removed by centrifugation, and the prepared GO / PEI was dissolved in 1 mL of deionized water for use.

[0025] PEI was used as a reducing agent and immobilization agent for the in situ generation and immobilization of gold nanoparticles on the GO / PEI surface. Add 2 mg HAuCl to the GO / PEI prepared in step (1) 4 ·3H 2 0, after shaking evenly, stir reaction 60min in 60 ℃ of water baths, until wine red gold nanometer appears. After the reaction is finished, the prepared nano-gold-modified graphene oxid...

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Abstract

The present invention relates to a glycopeptide enriched 4-mercaptophenylboronic acid immobilized graphene oxide composite nanometer material. According to the 4-mercaptophenylboronic acid immobilized graphene oxide composite nanometer material, polyethyleneimine modified graphene oxide is prepared according to an electrostatic interaction between graphene oxide and polyethyleneimine; the polyethyleneimine is adopted as a reducing reagent and an immobilizing reagent to produce and immobilize nanometer gold on the surface of the polyethyleneimine modified graphene oxide composite nanometer material in an in situ manner so as to prepare a nanometer gold modified graphene oxide composite nanometer material; and the nanometer gold modified graphene oxide composite nanometer material is adopted as a matrix to immobilize 4-mercaptophenylboronic acid in an ethanol solution through a self-assembly effect between nanometer gold and 4-mercaptophenylboronic acid, wherein the 4-mercaptophenylboronic acid immobilized graphene oxide composite nanometer material is adopted as a novel function nanometer material for glycopeptide enrichment. In the method, the graphene oxide composite nanometer material is firstly adopted as the matrix for 4-mercaptophenylboronic acid immobilization, and is successfully used for glycopeptide enrichment.

Description

technical field [0001] The invention relates to the preparation of a 4-mercaptophenylboronic acid-immobilized graphene oxide composite nanomaterial, which can be used for the specific enrichment of glycopeptides. Background technique [0002] Since its discovery in 2004, graphene has been increasingly studied as a new two-dimensional single-atom material. Graphene with a complete structure is a two-dimensional crystal composed of six-membered benzene rings without any unstable bonds. It has high chemical stability, its surface is in an inert state, and its interaction with other media is weak. There is a strong van der Waals force between the graphene and the sheet, which is easy to aggregate and makes it difficult to dissolve, which has caused great difficulties for the further research and application of graphene. In order to give full play to its excellent properties, effective chemical functionalization of graphene is necessary. As an important derivative of graphene, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/22B01J20/30C07K1/22
Inventor 张丽华江波曲焱焱杨开广张玉奎
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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