Method for inspecting acid-fast bacilli by liquid-based thin-layer cell smears

Abstract

The invention discloses a method for inspecting acid-fast bacilli by liquid-based thin-layer cell smears. The method comprises the following steps: collecting a specimen, and collecting 2-5ml of sputum of a subject; preparing a treating liquid, and preparing a sputum pretreating liquid for inspecting acid-fast bacilli by the liquid-based thin-layer cell smears; centrifuging, adding the sputum into the prepared sputum pretreating liquid, and vibrating for multiple seconds; preparing a section, absorbing multiple milliliters of the sputum at the bottom of a sputum pretreating liquid test tube by a sucker, putting the sputum into a section preparation bin, putting the section preparation bin into a section preparation machine, and taking out the section preparation bin to dry when the section preparation machine completes the preparation; and carrying out acid-fast staining and microscopic inspection. Compared with the prior art, the method provided by the invention has the advantages that the positive incidence for inspecting acid-fast bacilli by sputum thick smears is improved by tens of times; the tuberculosis infection source can be found out as soon as possible; the tuberculosis can be treated early if being found out early; the burden of patients is relieved; the tuberculosis expansion can be controlled.

Description

technical field [0001] The invention relates to a method for checking acid-fast bacilli, in particular to a method for checking acid-fast bacilli with a liquid-based thin-layer cell smear. Background technique [0002] Tuberculosis is a chronic infectious disease that seriously endangers human health. In tuberculosis control work, tuberculosis bacteriological examination is of great significance for discovering the source of infection, confirming diagnosis and formulating treatment plan, and is the gold standard for diagnosing tuberculosis. Tuberculosis bacteriological examination currently includes smear acid-fast staining microscopic examination and isolation culture. Microscopic examination of acid-fast staining of smears is a widely used technique in the bacteriological examination of tuberculosis worldwide. This method is simple in equipment and easy to operate, but the positive rate of detection is low (about 5%-10%), and it has shown a downward trend in recent years ...

AI Technical Summary

Problems solved by technology

Microscopic examination of acid-fast staining of smears is a widely used technique in the bacteriological examination of tuberculosis worldwide. This method is simple in equipment and easy to operate, but the positive rate of detection is low (about 5%-10%), and it has shown a downward trend in recent years

Separation culture is more sensitive and specific than smear acid-fast staining microscopic examination, but requires high equipment, complicated operation, long culture time, and it takes 2 months to report the results, which cannot meet the needs of clinical diagnosis

In order to break through the limitations of low positive rate of acid-fast staining microscopic examination of smears, long separation and culture time, and slow results, domestic and foreign have been discussing simple and practical inspection methods with high positive rate, but no successful reports have been found.