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Late blight resistance related protein, and related biomaterial and application thereof

A biomaterial and protein technology, applied in the field of late blight resistance-related proteins and related biomaterials, can solve problems such as high cost, impact on human health and environmental safety, and insensitivity to fungicides

Inactive Publication Date: 2014-05-28
INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is expensive and has certain negative impacts on human health and environmental safety
In addition, the rapid reproduction of pathogenic bacteria has resulted in some new physiological races being insensitive to commonly used fungicides
This disease is a big problem for organic growers who do not use any chemical pesticides in the production process

Method used

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  • Late blight resistance related protein, and related biomaterial and application thereof
  • Late blight resistance related protein, and related biomaterial and application thereof
  • Late blight resistance related protein, and related biomaterial and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Example 1. Cloning of SP-LBR3 gene and its functional verification

[0064] 1. Cloning of SP-LBR3 gene

[0065] Using the genomic DNA of tomato (Solanum lycopersicum) CLN2037B as a template, primers SP-LBR3EF3 (5'-taacctgcaggTTCAAACCATCTTCATAGAGGC-3') and SP-LBR3ER3 (5'-attggcgcgccTGGGGCTTAGAAAAAGGTTG-3') were prepared by using Phusion high-fidelity DNA polymerase (Thermo Fisher, Waltham, MA, USA) PCR amplification of SP-LBR3 gene upstream 4kb, SP-LBR3 gene and SP-LBR3 gene downstream 2kb fragment, this fragment is called SP-LBR3 gene expression cassette PCR product. The PCR product of SP-LBR3 gene expression cassette was cloned into pCR-Blunt II-TOPO (Invitrogen, Carlsbad, CA, USA) to obtain the recombinant vector pCR-SP-LBR3 containing the PCR product of SP-LBR3 gene expression cassette. The sequencing results show that the DNA sequence of the PCR product of the SP-LBR3 gene expression cassette is as shown in SEQ ID No.1. SEQ ID No.1 consists of 8007 nucleotides, and...

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Abstract

The invention discloses a late blight resistance related protein, and a related biomaterial and application thereof. The late blight resistance related protein is the protein as shown in a) or b): a) the protein comprising amino acid sequences as shown in SEQ ID No.2; b) the protein obtained by substituting and / or deleting and / or adding one or more amino acid residues from the amino acid sequences as shown in SEQ ID No.2 and related to the late blight resistance. The late blight resistance related protein gene can be transferred into a receptor of a tomato to obtain a transgenic tomato with the improved late blight resistance, so that the late blight resistance related protein gene is the gene related to the late blight resistance, and can be used to encode the protein related to the late blight resistance.

Description

technical field [0001] The present invention relates to late blight resistance-related proteins and related biological materials and applications. Background technique [0002] Late blight, caused by Phytophthora infestans, is one of the most devastating tomato field diseases. Under conditions conducive to the growth of the pathogen, late blight can spread at an alarming rate, killing the host in 7 to 10 days. Fungicides are currently the most commonly used method of controlling late blight. However, it is expensive and has a certain negative impact on human health and environmental safety. In addition, the rapid proliferation of pathogenic bacteria has resulted in some new physiological races that are not sensitive to commonly used fungicides. This disease is a big problem for organic growers who do not use any chemical pesticides in the production process. Therefore, the transfer of wild tomato disease resistance genes into cultivated species is an effective method to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82C12N1/15C12N1/19C12N1/21A01H5/00
CPCC07K14/415C12N15/8282
Inventor 郑峥刘磊李君明张春芝杜永臣
Owner INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
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