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Preparation method of carboxymethyl yeast glucan product and product prepared by the method

A technology of yeast glucan and carboxymethyl, which is applied in cosmetic preparations, preparations for skin care, cosmetics, etc., can solve the problems of complex secondary alkali addition process, poor safety of isopropanol, and large consumption of solvent, etc. Achieve excellent substitution effect, stable molecular weight, and the effect of saving reagents

Active Publication Date: 2016-11-30
ANGELYEAST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Isopropanol is less safe, and it consumes a lot of solvent when it is prepared by slurry method, and the process of adding alkali twice is complicated
It can be seen that there are certain shortcomings in the above methods

Method used

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  • Preparation method of carboxymethyl yeast glucan product and product prepared by the method
  • Preparation method of carboxymethyl yeast glucan product and product prepared by the method
  • Preparation method of carboxymethyl yeast glucan product and product prepared by the method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Weigh 100kg of yeast dextran and place it in a kneader, add 150kg of analytically pure NaOH, 250kg of 70% ethanol, and control the temperature at 25°C for 90min. Then 100kg of chloroacetic acid was added, and 150kg of 70% ethanol was added, and the temperature was maintained at 30°C for 90 minutes, and then the temperature was lowered to complete the reaction.

[0048] Take out the carboxymethyl dextran from the kneader, add 300kg of 70% ethanol, wash and centrifuge to get the precipitate. Repeat the washing and centrifugation for 3 times in the above-mentioned manner, adjust the pH to neutral with hydrochloric acid, collect the precipitate and dry it in an oven until the water content is 5.8%, crush it and pack it to obtain carboxymethyl yeast dextran powder product.

Embodiment 2

[0050] Put 100kg of yeast glucan in a kneader, add 50kg of analytical pure NaOH, 50kg of 85% ethanol, and control the temperature at 30°C for 50min. Then 30kg of chloroacetic acid was added, and 50kg of 85% ethanol was added, and the temperature was maintained at 80°C for 60 minutes, and then the temperature was lowered to complete the reaction.

[0051]Take out the carboxymethyl dextran from the kneader, add 1000kg of 85% ethanol, wash and centrifuge to get the precipitate. Repeat the washing and centrifugation 3 times in the above way, adjust the pH to neutral with hydrochloric acid, collect the precipitate and dissolve it in water, adjust the weight percentage concentration to 2%, filter it with diatomaceous earth until the OD (600nm) value is 0.3, and use ultraviolet light Visible spectrophotometer measures the OD value at 600nm, and directly packs the carboxymethyl yeast glucan liquid product.

Embodiment 3

[0053] Put 100kg of yeast dextran in a kneader, add 100kg of analytical pure NaOH, 200kg of 70% ethanol, control the temperature at 20°C for 60 minutes, then add 80kg of chloroacetic acid and 100kg of 70% ethanol, and keep the temperature at 70°C for 60 minutes Cool down to end the reaction.

[0054] Take out the carboxymethyl dextran from the kneader, add 800kg of 70% ethanol, wash and centrifuge to get the precipitate. Repeat the washing and centrifugation for 5 times in the above-mentioned manner, adjust the pH to neutral with hydrochloric acid, collect the precipitate, dry it in an oven until the water content is 5.1%, crush it and pack it to obtain carboxymethyl yeast dextran powder product.

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Abstract

The invention relates to a method for preparing a carboxymethyl yeast glucan product and a product prepared by the product. The preparation method comprises the following steps: adding 12.5 to 37.5 mol of hydroxide radicals per 1 kg of yeast glucan in the yeast glucan Add alkali according to the ratio of yeast dextran and ethanol, and add ethanol according to the mass ratio of yeast dextran and ethanol at 1:0.5~2.5, and react at 0~30°C; : 0.3~1.0, add chloroacetic acid, and add ethanol according to the mass ratio of yeast dextran and ethanol at 1:0.5~1.5 at the same time, react at 30~80°C, cool; add ethanol to the obtained solution, wash with hydrochloric acid at the same time And, washed, and centrifuged to obtain precipitated carboxymethyl yeast glucan. The carboxymethyl dextran product obtained by the present invention has a degree of substitution of 0.3-1.0, a molecular weight of 100-1000KD, is completely dissolved in water, and has moisturizing, anti-aging and other effects when applied in the field of cosmetics.

Description

technical field [0001] The invention relates to a method for preparing a carboxymethyl yeast glucan product and a product prepared therefrom. Background technique [0002] Yeast glucan is a functional polysaccharide existing in the cell wall of yeast. It has the effects of enhancing immunity, anti-infection, and anti-tumor. It can be used in food, cosmetics, animal nutrition and other fields. Dextran from yeast has a special three-dimensional helical structure and cannot be dissolved in water, thus limiting its application range in various fields. [0003] At present, there are reports in the literature that a large amount of isopropanol is used to prepare carboxymethyl dextran. In the process, a large amount of isopropanol is used as a solvent (slurry method), and the product prepared by adding alkali twice has a degree of substitution of 0.55. The degree of substitution refers to the average number of hydroxyl groups substituted by the reagent on each anhydroglucose unit,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/02A61K8/73A61Q19/08A61Q19/00
Inventor 张彦俞学锋李知洪余明华姚鹃张海波
Owner ANGELYEAST CO LTD
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