Preparation method and application of homological rumen micro-ecological regulating agent

Abstract

The invention relates to a preparation method and an application of a homological rumen micro-ecological regulating agent. The preparation method comprises the following steps: extracting rhizome dry power of China flower and derris fordii by 55% ethanol three times, concentrating the extracting solution to 1 / 20, and preparing a compound liquid in a certain proportion; further culturing a rumen fluid by the compound liquid to obtain a low-methane-activity lumen culture; and suspending the culture in artificial saliva and the compound liquid to prepare the homological rumen micro-ecological regulating agent. By respectively injecting the regulating agent two lumens of two types of cattle based on 0.01wt%, the lumen methane productions are respectively reduced by 23.44% and 21.45%, the total volatile fatty acids are improved by 12.53% and 19.47%, B / C are reduced by 35.21% and 37.71%, and microproteins are respectively improved by 12.43%-25.88% and 13.78%-26.4%. Methanogens and protozoa are respectively reduced by two orders of magnitudes, and fungi and bacteria are respectively improved by 1-2 orders of magnitudes.

Description

Technical field: [0001] The invention relates to a preparation method and application of a homologous rumen micro-ecological regulator for reducing rumen methane generation and improving rumen fermentation performance. Background technique: [0002] Methane (CH 4 ) is a powerful greenhouse gas with a greenhouse effect more than 20 times that of carbon dioxide. Ruminants are one of the main sources of methane gas, accounting for 15% of the total methane emissions, of which more than 90% comes from rumen anaerobic fermentation of ruminants. The methane produced by the rumen is also an important reason for the energy loss of ruminants. About 6% to 15% of the feed energy is lost in the form of methane during the rumen anaerobic fermentation of ruminants. Therefore, reducing the production of rumen methane can not only reduce the greenhouse gas emission of ruminants, but also improve the feed utilization efficiency of rumen and improve the production performance of ruminants. ...

AI Technical Summary

Problems solved by technology

When the natural product regulator enters the rumen, different microbial groups in the rumen interact, and through a series of biochemical processes such as degradation and transformation, the influence of the regulator may be weakened in a relatively short period of time, weakening the regulatory effect

However, when the heterologous microbial methane regulator enters the rumen, the native flora of the rumen will undergo a series of effects such as competition and repulsion, making it difficult for them to settle in the rumen, and it is even more difficult to form a stable population that can function

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