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Epothilone B separating and extracting method based on molecularly imprinted membrane filtering technology

A molecularly imprinted membrane and epothilone technology, applied in organic chemistry, fermentation, etc., to achieve good application prospects, facilitate industrialized large-scale production, and good selectivity

Active Publication Date: 2014-05-07
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] After searching, there is currently no relevant report on the application of molecularly imprinted polymer membranes to the separation and purification of epothilone B

Method used

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  • Epothilone B separating and extracting method based on molecularly imprinted membrane filtering technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Adsorption of the resin to the fermentation broth: Ferment S. cellulosus ATCC25532 in the liquid medium, and add the treated macroporous resin XAD-16 to the liquid medium. The macroporous resin XAD-16 exists in the whole In the fermentation process, the adsorption resin is obtained by filtering after 120 hours of fermentation;

[0027] Wherein, the addition amount of macroporous resin XAD-16 is 2% of the volume of liquid medium;

[0028] The macroporous resin XAD-16 is processed by the following method: First, soak the macroporous resin XAD-16 with 3 times the volume of the macroporous resin XAD-16 in methanol, shake the shaker for 12 hours, remove the methanol solution, and wash the macroporous resin with pure water XAD-16 until there is no smell of methanol; after soaking in methanol for 12 hours, wash with pure water until there is no smell of methanol;

[0029] The composition of the liquid medium is potato starch 2.5-3.0g / L, sucrose 0.7-1.0g / L, glucose 0.2-0.5...

Embodiment 2

[0038] (1) Adsorption of the resin to the fermentation broth: ferment S. cellulosus ATCC25569 in a liquid medium, and add the treated macroporous resin XAD-16 to the liquid medium, and the macroporous resin XAD-16 exists in the whole In the fermentation process, after 150 hours of fermentation, the adsorption resin is obtained by filtration.

[0039] Wherein, the addition amount of macroporous resin XAD-16 is 3% of the volume of liquid medium;

[0040] The macroporous resin XAD-16 is processed by the following method: First, soak the macroporous resin XAD-16 with 3 times the volume of the macroporous resin XAD-16 in methanol, shake the shaker for 12 hours, remove the methanol solution, and wash the macroporous resin with pure water XAD-16 until there is no smell of methanol; after soaking in methanol for 12 hours, wash with pure water until there is no smell of methanol;

[0041] The composition of the liquid medium is potato starch 2.5-3.0g / L, sucrose 0.7-1.0g / L, glucose 0.2...

Embodiment 3

[0050] (1) Adsorption of the resin to the fermentation broth: Ferment S. cellulosus ATCC25532 in a liquid medium, and add the treated macroporous resin XAD-16 to the liquid medium. The macroporous resin XAD-16 exists throughout the fermentation In the flow process, the adsorption resin is obtained by filtering after 130 hours of fermentation;

[0051] Wherein, the addition of macroporous resin XAD-16 is 5% of the volume of liquid medium;

[0052] The macroporous resin XAD-16 is processed by the following method: First, soak the macroporous resin XAD-16 with 3 times the volume of the macroporous resin XAD-16 in methanol, shake the shaker for 12 hours, remove the methanol solution, and wash the macroporous resin with pure water XAD-16 until there is no smell of methanol; after soaking in methanol for 12 hours, wash with pure water until there is no smell of methanol;

[0053] The composition of the liquid medium is potato starch 2.5-3.0g / L, sucrose 0.7-1.0g / L, glucose 0.2-0.5g / ...

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Abstract

The invention discloses an epothilone B separating and extracting method based on the molecularly imprinted membrane filtering technology. The method comprises the following steps: fermentation liquid is adsorbed by macroporous resin and resolved by ethyl acetate to obtain a crude product; the crude product is filtered by a micro-filtration membrane and an ultrafiltration membrane to remove macromolecules and other impurities; filtrate is filtered by the epothilone B molecularly imprinted membrane to obtain pure epothilone B liquid; and finally, the pure epothilone B liquid is obtained through crystallization. The method is convenient for continuous operation, is easy to enlarge, simple to operate, low in cost and high in selectivity, can rapidly separate the epothilone B molecules from a mixture of structural analogues, and can be put into industrial production easily.

Description

technical field [0001] The invention belongs to the field of antibiotic separation and extraction, and in particular relates to a method for separation and extraction of epothilone B based on molecular imprinted membrane filtration technology. Background technique [0002] Epothilone B (Epothilone B) is a class of secondary metabolites of macrolides produced by the microorganism S. cellulosus. In 1994, the National Cancer Society of the United States found that epothilone B has inhibitory activity on tumor cells. Merck laboratory found that it and pirobinol have at least the same effect in tubulin polymerization experiments, which proved that epothilone B has the same effect on tumor cells. Inhibitory effect, further research found that epothilone has superior anti-tumor properties than pirobinol, so researchers put more energy into its synthesis and separation and extraction, in order to develop it into antineoplastic drugs. However, the currently published methods for se...

Claims

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Application Information

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IPC IPC(8): C07D493/04
CPCC07D493/04C12P17/181
Inventor 龚国利赵婷峰
Owner SHAANXI UNIV OF SCI & TECH
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