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A method for establishing a mouse gastric cancer model

A gastric cancer model and a technology for establishing a method, applied in the experimental field, can solve the problems of lack of immune system and errors in immunodeficient mice, and achieve the effect of good stability and induction of chronic stress.

Inactive Publication Date: 2016-05-11
徐泽宽
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, current studies in the field of tumors have found that the microenvironment of the immune system of the body plays an important role in the formation of tumors, while immunodeficiency mice lack a normal immune system. Therefore, using this model for research is bound to produce errors

Method used

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  • A method for establishing a mouse gastric cancer model
  • A method for establishing a mouse gastric cancer model
  • A method for establishing a mouse gastric cancer model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Gastric cancer-inducing compound: MNU (n-methyl-n-nitrosourea, N-methyl-Nnitrosourea). Administration route: dissolved in drinking water of mice. Dose: 240ppm.

[0023] 2. Stress mold: 50ml centrifuge tube (poke 5-10 small holes)

[0024] 3. Steps:

[0025] Take a 4-week-old C57BL / 6 mouse, put the mouse into a 50ml centrifuge tube (poke 5-10 small holes), restrain its activities, put one mouse in each centrifuge tube, 6 hours a day, for 28 days It is a stress cycle, and each stress cycle is separated by 5 days, and there are 3 stress cycles in total. At the same time of stress, the mice were given drinking water containing MNU, and the administration was given one week apart, and normal drinking water was given between the administrations, for a total of 5 weeks. At the same time, the MNU group that only gave the mice drinking water containing MNU without stress treatment and the stress group that only received stress treatment according to the above method were ...

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Abstract

The invention discloses a building method of a mouse stomach cancer model. A four-week-old C57BL / 6 mouse is taken and placed in a tubular device, wherein movement of the mouse is restrained but the mouse can breathe freely in the tubular device; every stress period lasts for 25-30 days, and six to eight hours are needed every day; the interval between every two stress periods is 5-7 days, and two to three stress periods are needed; when stress is carried out, the mouse drinks water containing N-nitro compounds is provided for the mouse, medicine is fed for a week and is fed again after a week, normal drinking water is provided for the mouse at the medicine feeding intervals, and the mouse needs to be fed with medicine for five weeks; the mouse is bred for 35 weeks conventionally after the medicine feeding, and the mouse suffering the stomach cancer is obtained. According to prior literatures and reports, the stomach cancer formation rate obtained after MNU treatment is carried out on a C57BL / 6 mouse purely is about 30%; after the MNU treatment and the stress treatment are combined, the stomach cancer formation rate of the C57BL / 6 mouse increases to be 60%.

Description

technical field [0001] The invention belongs to the field of experiment technology, and relates to a method for establishing a mouse gastric cancer model. Background technique [0002] At present, mouse gastric cancer models are divided into spontaneous tumor models and transplanted tumor models. The xenograft tumor model uses immunodeficient mice (such as athymic nude mice, SCID mice), and tumor cells or tumor masses are transplanted into immunodeficient mice to form tumors. Gastric cancer spontaneous tumor models in mice can be further divided into genetically engineered mouse gastric cancer models and gastric cancer models stimulated by environmental factors. There are currently three genetically engineered mouse gastric cancer models in the world: INS-GAS mice, TFF-1knockout mice and RunnX3knockout mice. All these mice use the gene homologous recombination of mouse embryonic stem cells to knock out or knock in specific genes in the mouse genome. At present, environmen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61D3/00A61K31/17
Inventor 支小飞徐皓杨力徐泽宽
Owner 徐泽宽
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