Marrow chromosome extraction kit

A kit and chromosome technology, applied in the fields of life science and biology, can solve the problems of low mitosis index, poor dispersion, and unstable cell cycle of bone marrow chromosomes, and achieve good shape and dispersion, increased chromosome length, and G-band background clear effect

Active Publication Date: 2014-04-09
FUZHOU ADICON CLINICAL LAB INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the interference of a large number of fat particles in the bone marrow, the cell cycle of various cell lines in the bone marrow is not fixed, not uniform, and it is difficult to treat them differently, resulting in low chromosome division index, short and thick chromosomes, poor dispersion, and cost higher

Method used

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  • Marrow chromosome extraction kit
  • Marrow chromosome extraction kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1 Preparation of bone marrow cell medium

[0057] Incubine cell culture is added to the basic medium, penicillin / rinomanticin, prenatal serum and human lymphoma cell culture.The basic medium is the RPMI1640 medium.

[0058] The amount of each added ingredients is as follows:

[0059] Penicillin / chaincin 5-15ul / ml

[0060] Fetal cattle serum 60-140 ul / ml

[0061] Human lymphoma cell culture 60-140 UL / ML

[0062] Among them, penicillin can be selected from 10000U / ml, and chaincin can be selected from 10000 μg / ml.In other embodiments, it can be selected from other concentration of penicillin and splitterin to prepare a medium.

[0063] Among them, human lymphoma cell culture can be obtained or prepared in accordance with the existing methods. In this embodiment, the cells are used to recover and transparent.Cells and centrifugal, after centrifugal, collect clearing and filtering cell culture.

[0064] The more specific method of making human lymphoma cell culture inclu...

Embodiment 2

[0074] Example 2 Bone marrow chromosome extraction kit

[0075] Bone marrow chromosome extraction kit includes reagent 1. Reagent 1, reagent 2, reagent 3 and reagent 4 liquid reagent.

[0076] Reagent 1 is a bone marrow cell culture medium. It is prepared according to the method of Example 1. Reagent 1 includes basic medium and ingredients.

[0077] Penicillin / chaincin 5-15ul / ml

[0078] Fetal cattle serum 60-140 ul / ml

[0079] Human lymphoma cell culture 60-140 UL / ML

[0080] Preferably, reagent 1 includes basic medium and each additional ingredients.

[0081] Penicillin / chaincin 7-12ul / ml

[0082] Fetal Bull Serum 80-120 UL / ML

[0083] Human lymphoma cell culture 80-120 UL / ML

[0084] More preferably, reagent 1 includes basic medium and each additional ingredients.

[0085] Penicillin / chaincin 8ul / ml

[0086] Fetal Niu serum 96 ul / ml

[0087] Human lymphoma cell culture 96 UL / ML

[0088] Among them, the basic medium can be RPMI1640 medium.

[0089] The composition and concentrat...

Embodiment 3

[0114] Example 3 Bone marrow cell culture and preparation of chromosome specimens

[0115] The examples of this embodiment are cultivated in the following methods to cultivate bone marrow cells and dyeing systems.In other embodiments, other methods can also be used to cultivate bone marrow cells and dyeing systems.The method described in this example is:

[0116] (1) Configure bone marrow culture medium: formulate according to the method of Example 1;

[0117] (2) vaccination: in vaccination of bone marrow cells to the medium described in step 1;

[0118] (3) Termid training;

[0119] (4) Collection of bone marrow cell culture materials for dyeing systems;

[0120] (5) Chromatology specimen production: After dyeing, the chromosomal specimen with a gifer zone is obtained.

[0121] The medium and reagents required to cultivate bone marrow cells and chromosomes can be selected by the bone marrow chromosome extraction kit described in this Example 2, or it can be prepared by themselve...

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Abstract

A disclosed marrow chromosome extraction kit comprises a liquid reagent composed of reagent 1, reagent 2, reagent 3 and reagent 4, wherein the reagent 1 is a mixture of the following compositions: RPMI1640 medium, penicillin / streptomycin, fetal calf serum and mankind lymphoma cell cultures. G band prepared by employing a marrow chromosome suspension extracted by employing the kit has the following advantages that division phases are relatively more, time is saved and labor is saved; the length of chromosome in division phases is relatively long, and the band is relatively clear; the dispersity is relatively good and the analysis is facilitated; and the detection rate of abnormal chromosome is relatively high and the diagnosis result is relatively reliable. Therefore, the kit is relatively high in accuracy when applied to marrow chromosome karyotyping, and has relatively wide application prospect.

Description

Technical field [0001] The invention is the field of life sciences and biotechnology. It specially involves a kit for bone marrow chromosome extraction, which is used to analyze bone marrow chromosome. [0002] Background technique [0003] Because the chromosomes are mainly dyed by GIEMSA dyes, they are called G display technology, and the strap displayed by it is distributed on the entire chromosome.People will use various methods and different dyes to treat chromosomal specimens, and each chromosomes appear on the dark and dark, or the technologies with different shades of the shades are called banding technique.Since the 1970s, it has developed a lot of display technology, and among the many tape technologies (Q, G, C, C, R belt, T belt), the G belt is currently widely usedBelt. [0004] Studies have found that after the human chromosome specimen is treated by intease, NA0H, citric acid, or urea, givemsa is dyed, which can display the shades alternately on each chromosome, wh...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCG01N1/28G01N1/30G01N21/84
Inventor 程建兵夏成青陈红梅郭福晓生帅
Owner FUZHOU ADICON CLINICAL LAB INC
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