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Method for producing genetically modified wuzhishan pigs expressing fluorescent proteins

A fluorescent protein, Wuzhishan pig technology, applied in biochemical equipment and methods, genetic engineering, plant genetic improvement, etc., can solve problems such as low similarity and limited application of cell, tissue or organ transplantation

Inactive Publication Date: 2014-03-19
尹熙俊
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, the recipients of transgenic pigs produced in the related art have a low degree of similarity to human beings in their gene sequences, so the application of their cells, tissues or organs for transplantation is limited to a certain extent.

Method used

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  • Method for producing genetically modified wuzhishan pigs expressing fluorescent proteins
  • Method for producing genetically modified wuzhishan pigs expressing fluorescent proteins
  • Method for producing genetically modified wuzhishan pigs expressing fluorescent proteins

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Embodiment

[0044] Example: Method for producing transgenic Wuzhishan pig expressing red fluorescent protein

[0045] The method for producing transgenic Wuzhishan pig expressing fluorescent protein provided by the invention can be used to produce transgenic Wuzhishan pig expressing various fluorescent proteins such as red fluorescent protein, green fluorescent protein or blue fluorescent protein. In the embodiment of the present invention, the method of producing transgenic Wuzhishan pig expressing red fluorescent protein is adopted.

[0046] Step 1, obtaining ear fibroblasts of Wuzhishan pigs that can be transfected, can be specifically operated as follows:

[0047] The ear tissue of the donor pig was collected by aseptic operation, and conventional tissue blocks were used to inoculate and culture to obtain the ear fibroblasts of Wuzhishan pig.

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Abstract

The invention relates to the field of genetically modified animals, and in particular relates to a method for producing genetically modified wuzhishan pigs expressing fluorescent proteins. According to the method, the genetically modified wuzhishan pigs expressing red fluorescent proteins are produced by transfecting wuzhishan body cells by a fluorescent protein expression vector. Because the similarity of a gene sequence of the wuzhishan pig and a gene sequence of the human is up to more than 90 percent, the blood type of the wuzhishan pig is similar to the O-type blood of the human body, and the characteristics of the blood physiological and biochemical indexes, the metabolism of various tissue organs against medicines and the like are similar to those of the human, so that the transplantation of cells, tissues or organs is popularized and widely applied to medicines, raise livestock and veterinary medicine, particularly in various tests of human comparative medicine; moreover, the method provides the scientific evidence for the research of human organ transplantation, various kinds of animal model cultivation, development and application research on humanized pig organ transplantation.

Description

technical field [0001] The invention relates to the field of transgenic animals, in particular to a method for producing transgenic Wuzhishan pigs expressing fluorescent proteins. Background technique [0002] With the increasing application of cell, tissue and organ transplantation, animals and methods that can provide cells, tissues and organs for transplantation have received extensive attention and research. Among them, the use of genetic engineering to obtain transgenic animals, especially transgenic pigs capable of expressing fluorescence, has been widely studied. In recent years, transgenic pigs with different fluorescent genes have been successfully developed. [0003] In the related technology, by transferring the fluorescent protein gene into the pig body, the whole body of the transgenic pig expresses the fluorescent protein. When the cells, tissues or organs capable of expressing fluorescence of the transgenic pig are used for transplantation, the transplanted c...

Claims

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Application Information

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IPC IPC(8): C12N15/85C12N15/877A01K67/027
Inventor 尹熙俊康锦丹李所崔成哲王晓明李秀元
Owner 尹熙俊
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