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Isatis root vegetable proteins or polypeptides, extraction method thereof, and application thereof in antiviral drug

A plant protein and Banlangen technology, applied in the field of medicine, can solve the problems of backward production technology, limited product promotion and application, and difficulty in entering the international market, and achieve the effect of easy preparation and simple extraction method

Inactive Publication Date: 2014-03-19
SHENZHEN POLYTECHNIC
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Radix isatidis has complex components, its material basis and mechanism of action are not clear, its production technology is still relatively backward, its production is in an extensive state, its products have low technological content and low added value, which limits the promotion and application of products and makes it difficult to enter the international market

Method used

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  • Isatis root vegetable proteins or polypeptides, extraction method thereof, and application thereof in antiviral drug
  • Isatis root vegetable proteins or polypeptides, extraction method thereof, and application thereof in antiviral drug
  • Isatis root vegetable proteins or polypeptides, extraction method thereof, and application thereof in antiviral drug

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Embodiment Construction

[0046] The above content of the present invention will be further described in detail below in the form of embodiments, but it should not be understood that the scope of the above subject matter of the present invention is limited to the following embodiments. All technologies implemented based on the above content of the present invention belong to The scope of the invention.

[0047] See figure 1 The Isatis indigotica plant protein or polypeptide of the embodiment of the present invention has a molecular weight of 15-50 kDa (kilodaltons) and a purity of 50 to 90% (mass percentage), which is obtained by purification from Isatis indigotica. Among them, there are four bands of 15, 24, 26, and 34KDa, 24KDa is the trypsin band, and the others are isatis protein or polypeptide.

[0048] The following uses BCA method (bicinchoninic acid) to determine the content (mass percentage) of plant protein or polypeptide in Isatis indigotica.

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Abstract

The invention provides isatis root vegetable proteins or polypeptides, extraction method thereof, and application thereof in antiviral drug. The method comprises the following steps: crushing isatis root medicinal materials; dipping crushed isatis root medicinal material into deionized water for many hours and centrifugating the material; filtering clear liquid with ultrafilter membrane; freezing and drying; obtaining isatis root crude proteins or polypeptides whose molecular weights are 15-50 kDa; detecting and obtaining crude proteins or polypeptides whose mass percentage is 50-90% of isatis roots. The isatis root vegetable proteins or polypeptides are used for preparing antiviral drugs, which are used for preparing and treating viral disease and inflammation, and improving immunization.

Description

Technical field [0001] The invention relates to a type of plant protein extracted from the Chinese medicine Radix Isatidis, an extraction method thereof, and application thereof in antiviral drugs, belonging to the technical field of medicine. Background technique [0002] In recent years, SARS, avian influenza, and the H1N1 influenza virus have frequently broke out in my country and the world, causing great damage to human life and social economy. [0003] For a long time, people have used vaccines to prevent viruses and have achieved good results, but vaccines are only effective when they match the virus subtype. Viruses mutate quickly and outbreaks are unpredictable, while vaccine research and production are relatively lagging. [0004] In response to the drawbacks of vaccines, people have begun extensive research on antiviral drugs to combat the virus. Western antiviral drugs have the characteristics of quick effect, high curative effect, and accurate action. However, each type...

Claims

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Application Information

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IPC IPC(8): C07K14/415C07K1/36C07K1/34C07K1/30C07K1/18A61K38/16A61P31/12A61P31/14A61P31/16A61P29/00A61P37/04
CPCA61K38/00C07K14/415
Inventor 刘西京
Owner SHENZHEN POLYTECHNIC
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