Traditional Chinese medicine particle for preventing and treating avian influenza and improving chicken flock immunity and preparation method thereof
A technology of traditional Chinese medicine granules and avian influenza, which is applied in the fields of traditional Chinese medicine and animal husbandry and veterinary medicine, can solve the problems such as the preparation method of the extract that is not involved, and achieve the effects of improving the immune effect of chicken flocks, the speed of showing effect is fast, and the process is simple and efficient.
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Embodiment 1
[0029] (1) Take Echinacea purpurea, add 12 times the amount of 50% ethanol to reflux and extract 3 times, each time for 1 hour, recover ethanol under reduced pressure, concentrate to a solution with a relative density of 1.10 at 60°C, perform polyamide column chromatography, and wash with 50% ethanol Remove, collect the eluent, recover ethanol under reduced pressure, concentrate to a thick paste, vacuum dry, pulverize to obtain Echinacea purpurea extract.
[0030] (2) Take the honeysuckle leaf medicinal material, add 14 times the amount of 60% ethanol solution to reflux and extract 3 times, each time for 1 hour, filter, combine the filtrate, recover ethanol under reduced pressure, concentrate to a thick paste, vacuum dry, crush to obtain the honeysuckle leaf extract things.
[0031] (3) Take Radix Astragali, add 10 times the amount of water to reflux and extract 3 times, each time for 1 hour, concentrate under reduced pressure to a solution with a relative density of 1.10 at 6...
Embodiment 2
[0035] (1) Take Echinacea purpurea, add 12 times the amount of 50% ethanol to reflux and extract 3 times, each time for 1 hour, recover ethanol under reduced pressure, concentrate to a solution with a relative density of 1.10 at 60°C, perform polyamide column chromatography, and wash with 50% ethanol Remove, collect the eluent, recover ethanol under reduced pressure, concentrate to a thick paste, vacuum dry, pulverize to obtain Echinacea purpurea extract.
[0036] (2) Take the honeysuckle leaf medicinal material, add 14 times the amount of 60% ethanol solution to reflux and extract 3 times, each time for 1 hour, filter, combine the filtrate, recover ethanol under reduced pressure, concentrate to a thick paste, vacuum dry, crush to obtain the honeysuckle leaf extract things.
[0037] (3) Take Radix Astragali, add 10 times the amount of water to reflux and extract 3 times, each time for 1.5 hours, concentrate under reduced pressure to a solution with a relative density of 1.10 a...
Embodiment 3
[0040] (1) Take Echinacea purpurea, add 10 times the amount of 50% ethanol to reflux and extract 3 times, each time for 1 hour, recover ethanol under reduced pressure, concentrate to a solution with a relative density of 1.20 at 60°C, perform polyamide column chromatography, and wash with 50% ethanol Remove, collect the eluent, recover ethanol under reduced pressure, concentrate to a thick paste, vacuum dry, pulverize to obtain Echinacea purpurea extract.
[0041] (2) Take Radix Astragali, add 12 times the amount of water to reflux and extract twice, each time for 3 hours, concentrate under reduced pressure to a solution with a relative density of 1.20 at 60°C, add 95% ethanol until the alcohol content reaches 70%, centrifuge, and take Precipitation, vacuum drying, crushing to obtain Astragalus extract.
[0042] (3) Mix 42.6g of the above-mentioned Echinacea purpurea extract and 36.3g of Astragalus extract with auxiliary materials (sucrose and dextrin at a weight ratio of 1:1)...
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