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Tissue culture method for hemerocallis sempervirens araki

A tissue culture and Hemerocallis technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems that hinder the large-scale application of Evergreen Hemerocallis, and achieve the effect of facilitating multiplication and rapid supply

Active Publication Date: 2015-04-29
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the production survey, Evergreen Hemerocallis seldom grows under natural conditions and can reproduce by seeding. Generally, the "division method" is used for reproduction, and the annual reproduction coefficient is generally about 4 to 5 times, which seriously hinders the large-scale application of Evergreen Hemerocallis.

Method used

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  • Tissue culture method for hemerocallis sempervirens araki
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  • Tissue culture method for hemerocallis sempervirens araki

Examples

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Embodiment 1

[0035] Example 1. A tissue culture method of evergreen daylily, which is followed by the following steps:

[0036] 1) Take the evergreen Hemerocallis plant that grows vigorously and has no pests and diseases, peel off the outer 1-2 layers of old leaves, and take the 1-2cm long stem at the tip of the stem and put it in a gauze bag to rinse with sterile water and running water for 0.5-1 hour.

[0037] 2) After the stems washed with the above running water are routinely disinfected (ie 0.1%w / v HgCl 2 After 6 to 10 minutes of treatment, rinse with sterile water 5 to 8 times, and then absorb dry with sterile filter paper), peel off the stem tip with a length of 3 to 5 mm, and inoculate it on the inducing sterile seedling medium for cultivation; The conditions are: 16 hours light, light intensity 30-40μmol m -2 ·S -1 , The temperature is 27 ± 1 ℃; 8 hours dark culture, the temperature is 21 ± 1 ℃; the above light and dark culture alternately;

[0038] The medium for inducing sterile seedl...

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Abstract

The invention discloses a tissue culture method for hemerocallis sempervirens araki. The tissue culture method comprises the following steps: (1) washing taken stems of hemerocallis sempervirens araki plants by water; (2) after the stems are disinfected, picking off stem tips and inoculating the stem tips into an induction aseptic seedling culture medium to be cultured; (3) after aseptic seedlings in the step (2) grow to 1.0cm-2.0cm, cutting to obtain plantlets I; inoculating the plantlets I into an adventitious bud induction culture medium to be cultured; (4) after adventitious buds induced from the plantlets I grow to 1.0cm-3.0cm, cutting by taking 2-5 strains as a cluster to obtain clustered plantlets; inoculating the clustered plantlets to a proliferation culture medium to be cultured; (5) after the adventitious buds on the clustered plantlets grow to 3.0cm-6.0cm, cutting to obtain plantlets III; inoculating the plantlets III into a rooting culture medium to be cultured; and (6) when at least five roots with the length of more than 2cm grow in the plantlets III, so as to obtain germchits capable of being planted out of a bottle.

Description

Technical field [0001] The present invention relates to a tissue culture method of evergreen daylilies. Background technique [0002] Evergreen Hemerocallis has the characteristics of evergreen in four seasons, long flowering period, strong disease resistance, cold tolerance, half shade tolerance, etc., and it is a popular green garden cultivation plant. In addition, the root of Evergreen Hemerocallis can be used as medicine, which has diuretic and cooling blood effects, and is used to treat edema, dysuria, drenching turbidity, jaundice, blood in the stool, and uterine bleeding. Anthraquinones, dihydrofuran-γ-lactams and other compounds isolated from Evergreen Hemerocallis have antibacterial, anticancer, and insecticidal activities. According to production surveys, evergreen Hemerocallis seldom grows under natural conditions to set seeds and reproduce. Generally, the "ramification method" is used for reproduction. The annual reproduction coefficient is generally about 4 to 5 tim...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 毛碧增刘辉辉
Owner ZHEJIANG UNIV
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