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Preparation method of titanium alloy surface growth factor composite coating

A growth factor and composite coating technology, applied in medical science, prostheses, etc., can solve the problems of direct immobilization of growth factor Nanogel, and achieve good activity retention, mild reaction conditions, and simple reaction operations.

Inactive Publication Date: 2015-04-29
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when modifying titanium alloy materials, due to the inertness of the titanium alloy itself and the steric hindrance effect caused by the large size of the growth factor Nanogel, it is difficult to directly immobilize the growth factor Nanogel on the surface of the titanium alloy.

Method used

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  • Preparation method of titanium alloy surface growth factor composite coating
  • Preparation method of titanium alloy surface growth factor composite coating

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] a. Dissolve dopamine in 20mM PBS solution with pH=7.4 to prepare a dopamine solution with a concentration of 1mg / ml, flush the above solution with nitrogen flow for 1h; 1-6mol / LN a Soak in OH for 30min, then wash with ultrapure water until the eluent is neutral; vacuum-dry the obtained Ti sheet; add the treated titanium sheet to the above dopamine solution, and then add 1mg of ammonium persulfate (APS) , reacted at 30°C for 2h;

[0024] b. Wash the titanium sheet obtained in a with ultrapure water, then soak it in 1mg / ml aqueous solution of avidin, and react at 20°C for 1h;

[0025] c. Dissolve the growth factor nanogel in 50mM boric acid buffer solution with pH=8.0 to prepare a 0.1mg / ml solution, take 1ml of the above solution and add 1ul10mg / ml Biotin-PEG-NHS solution, and magnetically stir for 2h;

[0026] d. Wash the titanium sheet obtained from the treatment in b with ultrapure water, and dry naturally under nitrogen flow. Take 10ul of the solution obtained in c...

Embodiment 2

[0029] a. Dissolve dopamine in 20mM PBS solution with pH=7.4 to prepare a dopamine solution with a concentration of 2mg / ml, flush the above solution with nitrogen flow for 2h; 3mol / L N a Soak in OH for 40min, then wash with ultrapure water until the eluent is neutral; vacuum-dry the obtained Ti sheet; add the treated titanium sheet to the above dopamine solution, and then add 2mg of ammonium persulfate (APS) , reacted at 40°C for 4h;

[0030] b. Wash the titanium sheet obtained in a with ultrapure water, then soak it in 2mg / ml aqueous solution of avidin, and react at 30°C for 12h;

[0031] c. Dissolve the growth factor nanogel in 50mM boric acid buffer solution with pH=8.0 to prepare a 0.5mg / ml solution, take 1ml of the above solution and add 5ul10mg / ml Biotin-PEG-NHS solution, magnetic stirring for 4h;

[0032] d. Wash the titanium sheet obtained from the treatment in b with ultrapure water, and dry naturally under nitrogen flow. Take 50ul of the solution obtained in c and...

Embodiment 3

[0035] a. Dissolve dopamine in 20mM PBS solution with pH=7.4 to prepare a dopamine solution with a concentration of 4mg / ml, and pour the above solution into a nitrogen flow for 4h; polish the surface of the Ti sheet, and then wash it in n-hexane and 6mol / L N a Soak in OH for 60min, then wash with ultrapure water until the eluent is neutral; vacuum-dry the obtained Ti sheet; add the treated titanium sheet to the above dopamine solution, and then add 4mg of ammonium persulfate (APS) , 30-60°C for 6 hours;

[0036]b. Wash the titanium sheet obtained in a with ultrapure water, then soak it in 4mg / ml aqueous solution of avidin, and react at 40°C for 24h;

[0037] c. Dissolve the growth factor nanogel in 50mM boric acid buffer solution with pH=8.0 to prepare a 1mg / ml solution, take 1ml of the above solution and add 10ul10mg / ml Biotin-PEG-NHS solution, and magnetically stir for 6h;

[0038] d. Wash the titanium sheet obtained from the treatment in b with ultrapure water, and dry na...

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Abstract

The invention relates to a preparation method of a titanium alloy surface growth factor composite coating. The preparation method comprises the steps of connecting growth factor nanogel and a target factor Biotin-PEG-NHS; then, treating the surface of a titanium alloy by using dopamine, and coupling Avidin onto the surface of the titanium alloy through the auto-agglutination action of the dopamine; then, fixing the growth factor nanogel on the surface of the titanium alloy through the special biological affinities of Biotin and Avidin, wherein growth factors include a bone morphogenetic protein (BMP), a transforming growth factor, a vascular endothelial growth factor, a platelet derived growth factor or fibroblast growth factor. The preparation method has the advantages of simplicity in reaction operation because of coupling the growth factor nanogel on the surface of the titanium alloy through simple one-pot reaction, mild in reaction condition and favorable in activity retention for a BMP-2.

Description

technical field [0001] The invention relates to the field of biomedical metal composite materials. The growth factor nanogel is connected with the targeting factor Biotin-PEG-NHS, and then the surface of the titanium alloy is treated with dopamine, and the affinity is induced by free radicals of dopamine. Avidin is coupled to the surface of titanium alloy, and then the growth factor nanogel is fixed to the surface of titanium alloy through the specific bioaffinity of biotin and Avidin, so as to realize the preparation of a new biomedical bone substitute material Background technique [0002] With the aging of the population and the younger trend of senile degenerative diseases, the incidence of various spine and joint diseases is increasing year by year, and the number of spinal fusion surgery and joint replacement surgery is also increasing. Titanium alloy has excellent mechanical properties and good biocompatibility. As the main material, titanium alloy has been widely use...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/40A61L27/22A61L27/06A61L27/54
Inventor 昌卫元原续波
Owner TIANJIN UNIV
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