Type 2 diabetes susceptibility genetic locus as well as detection method and kit thereof

A type 2 diabetes, kit technology, applied in the fields of molecular biology and medicine, can solve the reports that do not confirm the correlation between GRK5 gene or RASGRP1 gene and type 2 diabetes, the risk allele effect value is small, and there is no confirmation that SNP is related to type 2 diabetes. Type 2 diabetes related reports and other issues

Inactive Publication Date: 2014-01-22
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the risk alleles of these susceptibility genes have very small effect sizes, and the overall effect size only explains 10-15% of the heritability of type 2 diabetes.
[0008] Although there are many studies on various gene polymorphisms and type 2 diabetes, there is no report on the correlation between GRK5 gene or RASGRP1 gene and type 2 diabetes, and there is no confirmation that the GRK5 gene or RASGRP1 gene SNP of the present invention is related to Type 2 diabetes related reports

Method used

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  • Type 2 diabetes susceptibility genetic locus as well as detection method and kit thereof
  • Type 2 diabetes susceptibility genetic locus as well as detection method and kit thereof
  • Type 2 diabetes susceptibility genetic locus as well as detection method and kit thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0105] Identification of SNPs associated with type 2 diabetes

[0106] 1.1 Study population

[0107] The inventors carried out a genome-wide association analysis study (GWAS) of type 2 diabetes in several independent population samples, and the study included 3 research periods. A total of 1,999 diabetic patients and 1,976 non-diabetic controls were included in the population samples used for the first phase of genome-wide microarray analysis. The sample comes from the following population surveys: "Nutrition and Health Status Survey of Middle-aged and Elderly People in China (NHAPC)" (312 cases and 815 controls), "Gut Microbiota and Obesity Study (GMOS)" (82 cases and 163 Control), "Fudan Huashan Study (FDHS)" (807 cases and 339 controls), and "Beijing Diabetes Survey (BDS)" (798 cases and 659 controls). A total of 13,517 unrelated Chinese Hans (6,570 diabetic patients and 6,947 non-diabetic controls) were included in the sample used to validate the 96 T2DM loci in the seco...

Embodiment 2

[0143] Type 2 Diabetes Susceptibility Detection Kit

[0144] As described in Example 1, the presence of rs10886471 of the GRK5 gene: that is, the 134th C→T mutation in SEQ ID NO.: 1 is closely related to type 2 diabetes. Therefore, based on this mutation, GRK5 gene-specific primers can be designed to amplify and detect using the patient's DNA as a template.

[0145] Prepare a test kit (100 person-times), which contains:

[0146]

[0147] A test group composed of 100 people was randomly selected, including subjects who were unknown whether they had type 2 diabetes, patients who were known to have type 2 diabetes, and normal people who had been tested without type 2 diabetes.

[0148] Draw 3ml of peripheral blood from the subject to be tested in the test group, and use conventional methods (or use a specific kit) to extract DNA from the blood. Dilute the PCR primers in the type 2 diabetes detection kit to 1μmol / μl, and use the extracted DNA as a template to perform PCR reac...

Embodiment 3

[0154] Auxiliary testing for susceptibility to type 2 diabetes

[0155] Repeat the detection of Example 2, the difference is that 80 people are randomly selected (whether they have symptoms of type 2 diabetes before the detection) for detection.

[0156] Prepare a test kit (100 person-times), which contains:

[0157]

[0158] Draw 3ml of peripheral blood from the subject to be tested, and use conventional methods (or use a specific kit) to extract DNA from the blood. Dilute the PCR primers in the type 2 diabetes detection kit to 1μmol / μl, and use the extracted DNA as a template to perform PCR reaction with the provided primers. After the PCR product was purified, it was sequenced with an ABI 3730 DNA sequencer, and the sequence was read and SNP confirmed with Polyphred software.

[0159] The results also confirmed that the proportion of type 2 diabetes in the test subjects containing the 134th C site in rs10886471SEQ ID NO.:1 was significantly higher than that of the test...

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Abstract

The invention relates to a type 2 diabetes susceptibility genetic locus as well as a detection method and a kit of the type 2 diabetes susceptibility genetic locus. Particularly, the invention discloses the type 2 diabetes susceptibility genetic locus found on the basis of genome-wide association study. The invention also provides the method for detecting the type 2 diabetes susceptibility. The method comprises steps of detecting individual GRK5 gene and RASGRP1 gene, and determining whether the transcript and/or protein has variation compared with normal transcript and/or protein, if yes, the individual is more likely to be attacked by the type 2 diabetes than normal people. The invention also discloses the corresponding kit for detection.

Description

technical field [0001] The present invention relates to the fields of molecular biology and medicine. More specifically, it relates to the single nucleotide polymorphism (single nucleotide polymorphism, SNP) of GRK5 gene and RASGRP1 gene and their correlation with type 2 diabetes. The present invention also relates to methods and kits for detecting these SNPs. Background technique [0002] Diabetes mellitus is a group of syndromes composed of different pathophysiological changes characterized by abnormal glucose metabolism. There is great heterogeneity among different patients. The two main pathophysiological changes are insufficient insulin secretion and insulin resistance. [0003] About 92 million adults now have type 2 diabetes. Although changes in nutritional status and lifestyle, and the increase in the incidence of obesity are important factors leading to the increase in the incidence of type 2 diabetes, genetic factors also play an indispensable role. In the past ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6883C12Q2600/156
Inventor 林旭黎怀星甘蔚王一钦鲁玲
Owner SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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