Silkworm chrysalis active polypeptide after-sun repairing gel and preparation method thereof
A technology of silkworm chrysalis active polypeptide and after-sun repair, which is applied in the field of skin care products to achieve the effect of eliminating allergic factors
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Embodiment 1
[0087] (1) Preparation of silkworm chrysalis active polypeptide:
[0088] (1) Prepare a silkworm chrysalis protein solution with a concentration of 12% with deionized water, add papain, the enzyme amount is 20000IU / g silkworm chrysalis protein, enzymolysis temperature is 55°C, pH value is 7.0, enzymolysis time is 3h, and 92 ℃ inactivate the enzyme for 13 minutes;
[0089] (2) After the first step of enzymatic hydrolysis is completed, adjust the pH of the enzymatic hydrolysis solution to 9.0, add Esperase protease, the amount of enzyme added is 0.15KNPU / g silkworm chrysalis protein, enzymatic hydrolysis for 3 hours, the enzymatic hydrolysis temperature is 55°C, and the enzyme is extinguished at 92°C after the enzymatic hydrolysis is completed. Enzyme 13min;
[0090] (3) Filtrate the insoluble matter from the material after deactivating the enzyme to obtain a light yellow, clear and transparent solution. The polypeptide solution is passed through an ultra-microfiltration membra...
Embodiment 2
[0119] (1) Preparation of silkworm chrysalis active polypeptide:
[0120](1) Prepare a silkworm chrysalis protein solution with a concentration of 10% in deionized water, add papain, the enzyme amount is 8000IU / g silkworm chrysalis protein, the enzymolysis temperature is 55°C, the pH value is 7.0, the enzymolysis time is 3h, and the enzymolysis time is 92 ℃ inactivate the enzyme for 13 minutes;
[0121] (2) After the first step of enzymatic hydrolysis is completed, adjust the pH of the enzymatic hydrolysis solution to 9.0, add Esperase protease, the amount of enzyme added is 0.075KNPU / g silkworm chrysalis protein, enzymatic hydrolysis for 3 hours, the enzymatic hydrolysis temperature is 55°C, and the enzyme is extinguished at 92°C after the enzymatic hydrolysis is completed. Enzyme 13min;
[0122] (3) Filtrate the insoluble matter from the material after deactivating the enzyme to obtain a light yellow, clear and transparent solution. The polypeptide solution is passed throug...
Embodiment 3
[0130] (1) Preparation of silkworm chrysalis active polypeptide:
[0131] (1) Prepare a silkworm chrysalis protein solution with a concentration of 15% in deionized water, add papain, the enzyme amount is 60000IU / g silkworm chrysalis protein, enzymolysis temperature is 55°C, pH value is 7.0, enzymolysis time is 3h, and the enzymolysis time is 92 ℃ inactivate the enzyme for 13 minutes;
[0132] (2) After the first step of enzymatic hydrolysis is completed, adjust the pH of the enzymatic hydrolysis solution to 9.0, add Esperase protease, the amount of enzyme added is 0.225KNPU / g silkworm chrysalis protein, enzymatic hydrolysis for 3 hours, the enzymatic hydrolysis temperature is 55°C, and the enzyme is extinguished at 92°C after the enzymatic hydrolysis is completed. Enzyme 13min;
[0133] (3) Filtrate the insoluble matter from the material after deactivating the enzyme to obtain a light yellow, clear and transparent solution. The polypeptide solution is passed through an ultra...
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