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Composition of peony seed total stilbene compounds as well as extraction method and application thereof

An extraction method and compound technology, which are applied in the fields of application, medical preparations containing active ingredients, food science, etc., can solve the problems of low yield of stilbene compounds, toxic organic solvents, difficult industrialization, etc., and achieve economical simplicity and safety. Good sex, good effect against Gram-positive bacteria and negative bacteria

Active Publication Date: 2015-05-13
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In recent years, research groups have studied stilbenes in the seeds of medicinal plants peony: Literature [He Chunnian. Research on the Genetics of Paeonia Medicinal Plants, China Xiehe Medical University [D], 2010] reported the separation process of 9 stilbene compounds, the yield of the obtained stilbene compounds was very low, and the monomer stilbene compounds obtained from 4 kg of raw materials were at most more than 100 mg. Difficult to industrialize
In addition, the separation material used is peony seeds, and there are still problems in the application of toxic organic solvents in the research process.

Method used

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  • Composition of peony seed total stilbene compounds as well as extraction method and application thereof
  • Composition of peony seed total stilbene compounds as well as extraction method and application thereof
  • Composition of peony seed total stilbene compounds as well as extraction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0090] A method for extracting a composition of total stilbenes of peony seeds, comprising the following steps:

[0091] 1) Take the raw material of Fengdan peony seeds, remove impurities, wash them twice with clean water, then place them in a constant temperature drying oven, dry them at 60°C for 3 hours, add them to a sheller to peel them after drying, and then peel the obtained peonies Dry the seed skins until the water content does not exceed 10%, crush them, take 100g and put them in an extractor, add ethanol with a mass concentration of 60% that is 10 times the weight of the peony seed skins, and reflux extraction at 40°C for 8 hours. Filtration, repeating the extraction and filtration process three times, combining the filtrate, evaporating and concentrating the filtrate obtained by filtration to obtain an extract, which is set aside;

[0092] 2) Dissolve the extract obtained in step 1) with 3 times its weight in acetone solvent, remove the insoluble matter, and then ad...

Embodiment 2

[0105] A method for extracting a composition of total stilbenes of peony seeds, comprising the following steps:

[0106] 1) Take the raw material of purple peony seeds, remove impurities, wash them with water for 3 times, then place them in a constant temperature drying oven, dry them at 80°C for 9 hours, add them to a sheller after drying, and peel the obtained peony seeds Dry the bark until the water content does not exceed 10%, crush it, take 300g and put it in an extractor, add ethanol with a mass concentration of 80% that is 20 times the weight of the peony seed bark, reflux and extract at 60°C for 10 hours, and filter after the extraction is completed , repeating the extraction and filtering process five times, merging the filtrate, and evaporating and concentrating the filtrate obtained by filtering to obtain an extract, which is set aside;

[0107] 2) Dissolve the extract obtained in step 1) with an ethanol solvent 5 times its weight, remove the insoluble matter, and t...

Embodiment 3

[0120] A method for extracting a composition of total stilbenes of peony seeds, comprising the following steps:

[0121] 1) Take the mixed raw materials of peony seeds and peony seeds with a mass ratio of 1:1, remove impurities, wash with water for 3 times, then place them in a constant temperature drying oven, and dry them at 70°C for 6 hours. Add in the peeling machine to peel off, then dry the obtained peony seed skin to a water content of no more than 10%, pulverize, get 100g and place it in an extractor, add 70% ethanol with a mass concentration of 15 times the weight of the tree peony skin, Reflux extraction at 50°C for 9 hours, filter after the extraction, repeat the extraction and filtration process six times, combine the filtrate, evaporate and concentrate the filtered filtrate to obtain an extract, and set aside;

[0122] 2) Dissolve the extract obtained in step 1) with ethyl acetate solvent 4 times its weight, remove the insoluble matter, and then add an adsorbent w...

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Abstract

The present invention relates to a compound composition of total stilbenes of tree peony seeds and its extraction method and application. The peony seed skin is extracted with ethanol under reflux, filtered and concentrated to obtain an extract, and then the extract is dissolved and mixed with an adsorbent, and then the mixture is sequentially It is extracted with a solvent, finally refined with acetone and activated carbon, and concentrated until there is no solvent to obtain the total stilbene compound composition. The 6 compounds are paeonol A, paeonol, paeonol C, cis-glucuronin, trans-glucuronin and maimateng H. The total stilbene compound composition of Moutan of the present invention has been proved by pharmacological tests to have good activity against Gram-positive and negative bacteria, and can be used as an antibacterial drug active ingredient alone or in combination with suitable excipients, etc. , as a food additive for food preservation, or for the preparation of antibacterial drugs.

Description

technical field [0001] The invention mainly relates to a composition of total stilbene compounds in peony seeds, an extraction method and application thereof. Background technique [0002] Stilbeniods refer to the general term of monomers and their polymers with 1,2-stilbene skeleton. Oligostilbenes belong to phenolic acid compounds, which have a wide range of biological activities, such as antibacterial and antifungal activities, anti-inflammatory activities, antioxidant activities, anti-tumor activities, anti-AIDS activities and hepatoprotective activities. [Li Xiaomei, Li Na, Huang Kaisheng, Lin Mao. Spectral characteristics, biosources and biological activities of resveratrol oligomer analogues, Acta Pharmaceutica Sinica, 2002, 37(1): 69-74; Chen Li, Chen Jianbo. Stilbene compounds A review of pharmacological research in Guangdong Pharmaceutical Sciences, 2005,15(3):84-86] [0003] Peony seed oil contains a variety of unsaturated fatty acids, especially rare odd-number...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/343A61P31/04A23L3/3544
Inventor 刘普邓瑞雪付改玲杨丹蕾刘欣高嘉屿尹卫平柴元武
Owner HENAN UNIV OF SCI & TECH
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